Complementation of Replication-deficient Deletion Derivatives of Plasmid Mini-F

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Sep 1

PMID 6268604

Citations 6

Authors

J Ebbers

R Eichenlaub

Affiliations

Soon will be listed here.

Abstract

Deleted mini-F plasmids with defects in replication were constructed and tested to see whether they could be rescued through complementation by a helper plasmid. This allowed us to identify two genetic loci determining trans-acting functions required for stable maintenance of plasmid mini-F, one encoded by the PstI fragment from 45.7 to 47.3 F-coordinates (F) and the other most probably located in the region from 43.1 to 43.8 F. The smallest mini-F plasmid that could be established through complementation consists of the PstI fragment 44.0 to 45.7 F, encoding origin II and the incB locus.

Citing Articles

Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Komai N, Nishizawa T, Hayakawa Y, Murotsu T, Matsubara K Mol Gen Genet. 1982; 186(2):193-203.

PMID: 7050629 DOI: 10.1007/BF00331850.

Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment.

Tolun A, Helinski D Mol Gen Genet. 1982; 186(3):372-7.

PMID: 6750320 DOI: 10.1007/BF00729456.

Regulated expression of a gene important for replication of plasmid F in E. coli.

Sogaard-Andersen L, Rokeach L, Molin S EMBO J. 1984; 3(2):257-62.

PMID: 6325163 PMC: 557332. DOI: 10.1002/j.1460-2075.1984.tb01794.x.

Cloning of the pif region of the F sex factor and identification of a pif protein product.

Rotman G, Cooney R, MALAMY M J Bacteriol. 1983; 155(1):254-64.

PMID: 6305912 PMC: 217676. DOI: 10.1128/jb.155.1.254-264.1983.

Cloning and analysis of pif, replication and leading regions of the F plasmid.

Jackson R, Cram D, Ray A, DiBerardino D, Skurray R Mol Gen Genet. 1984; 197(1):129-36.

PMID: 6096669 DOI: 10.1007/BF00327933.

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