Analysis of RpsD Mutations in Escherichia Coli. IV. Accumulation of Minor Forms of Protein S7(K) in Ribosomes of RpsD Mutant Strains Due to Translational Read-through

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Feb 1

PMID 6990204

Citations 7

Authors

M O Olsson

L A ISAKSSON

Affiliations

Soon will be listed here.

Abstract

A certain proportion of protein S7 exists in an altered form in E. coli rpsD (S4) mutants. Depending on the type of S4 mutation involved, two different forms of the altered S7 can be distinguished. The unusual form is longer than normal S7 by about 500 daltons due to extra material at the carboxyl end of the protein. It is suggested that a mutationally altered S4 might lower the efficiency of termination during translation of the messenger for S7. This results in an increased frequency of translational read-through, which gives the observed longer forms of S7. Data are interpreted to mean that one class of S4 mutants might suppress UGA and UAG whereas another class only suppresses UGA.

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