Synthesis in E. Coli of a Polypeptide with Human Leukocyte Interferon Activity

Overview

Journal Nature

Specialty Science

Date 1980 Mar 27

PMID 6987533

Citations 107

Authors

S Nagata

H Taira

A Hall

L Johnsrud

M Streuli

J Ecsodi

W Boll

K Cantell

C Weissmann

Affiliations

Soon will be listed here.

Abstract

Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.

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