In Vitro Synthesis of IgE by Human Peripheral Blood Leucocytes. II Modulation of IgE B Cell Activity by Isotype-specific Regulatory T Cells

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1981 Sep 1

PMID 6978205

Citations 8

Authors

G H Holt

K J Turner

B J Holt

Affiliations

Soon will be listed here.

Abstract

IgE and IgG synthesis was demonstrated in cultures of isolated B cells from normal subjects, and those manifesting mild to severe atopy. Titration of autochthonous T cells into B cell cultures revealed both IgE isotype-specific T-help and T-suppression at low and high T:B ratios respectively. Increasing levels of help for IgG synthesis usually accompanied the stepwise addition of the same T cells to autochthonous or allogeneic B cells cultures. Experiments comparing the activity of putative IgE suppressor T cells in autochthonous versus allogeneic B cell cultures indicated that allogeneic interactions per se selectively inhibit IgE B cells. This finding seriously questions earlier conclusions of a causal relationship between suppressor cell deficiency and the expression of the allergic phenotype.

Citing Articles

Functional assessment of T and B lymphocytes in patients with selective IgM deficiency.

de la Concha E, Garcia-Rodriguez M, Zabay J, Laso M, Alonso F, Bootello A Clin Exp Immunol. 1982; 49(3):670-6.

PMID: 6983403 PMC: 1536738.

In vitro synthesis of IgE by human peripheral blood leucocytes. III. Release of pre-formed antibody.

Turner K, Holt P, Holt B, Cameron K Clin Exp Immunol. 1983; 51(2):387-94.

PMID: 6839547 PMC: 1536902.

Selective enhancement of human IgE production in vitro by synergy of pokeweed mitogen and mercuric chloride.

Kimata H, Shinomiya K, MIKAWA H Clin Exp Immunol. 1983; 53(1):183-91.

PMID: 6223732 PMC: 1535527.

In vitro synthesis of IgE by human peripheral blood leucocytes: V. Functional heterogeneity within the IgE-B-cell pool.

Turner K, Holt P, Holt B, Cameron K, Hobday J, Thompson P Clin Exp Immunol. 1987; 68(2):409-17.

PMID: 3498575 PMC: 1542731.

Regulation of rat IgE responses by immune complexes.

HALL E, Gault E Immunology. 1987; 61(4):415-9.

PMID: 3443450 PMC: 1453430.

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