Selective Enhancement of Human IgE Production in Vitro by Synergy of Pokeweed Mitogen and Mercuric Chloride

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1983 Jul 1

PMID 6223732

Citations 5

Authors

H Kimata

K Shinomiya

H MIKAWA

Affiliations

Soon will be listed here.

Abstract

IgE production in vitro was investigated in cultures of human peripheral blood mononuclear cells (MNC) from non-atopic donors with pokeweed mitogen (PWM), mercuric chloride (HgCl2), or both. PWM alone induced a few IgE immunoglobulin secreting cells (ISC) detected by reverse plaque forming cells (PFC) and many IgG, IgM, and IgA PFC. HgCl2 alone failed to produce significant numbers of ISC of any class. PWM plus HgCl2 caused a selective increase of IgE PFC without affecting IgG, IgM, and IgA PFC. Co-cultures of B cells plus mitomycin C (MMC) treated T cells stimulated by PWM alone produced more IgG, IgM, IgA and IgE PFC than those of B cells plus T cells. However, PWM plus HgCl2 produced significantly more IgE PFC selectively in those cultures. This effect was observed in the cells of most of the donors, but a few donors showed different responses.

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