Mobility of Surface Proteins on Normal Rat Macrophages and on a "macrophagelike" Rat Tumor

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1981 Sep 1

PMID 6974736

Citations 1

Authors

B A Woda

J Yguerabide

J D FELDMAN

Affiliations

Soon will be listed here.

Abstract

Peritoneal macrophages endocytosed their histocompatibility antigens (RT1), Fc receptors (FcR), and concanavalin A (Con A) receptors after cross-linking by ligands, but did not cap these membrane proteins. The 323N cell, a "macrophage like" tumor cell, under identical conditions capped its surface proteins. Experiments measuring fluorescence recovery after photobleaching showed that the mobile fraction of RT1 was significantly greater in 323N cells than in normal peritoneal macrophages. Presumably, the membrane proteins of 323N are not as tethered to the cytoskeleton, or, if so, are in a nexus that is not the same as that which occurs between membrane proteins of normal macrophages and the cytoskeleton. The mobility of RT1 on normal lymphocytes was also different from that of macrophages. These observations suggest that the movement of membrane molecules is determined by cell type and is regulated by the cytoskeleton which varies in structure and function from cell type to cell type.

Citing Articles

Fc receptor modulation in mononuclear phagocytes maintained on immobilized immune complexes occurs by diffusion of the receptor molecule.

Michl J, Pieczonka M, Unkeless J, Bell G, Silverstein S J Exp Med. 1983; 157(6):2121-39.

PMID: 6343550 PMC: 2187058. DOI: 10.1084/jem.157.6.2121.

References

Poste G, Papahadjopoulos D, Jacobson K, Vail W . Local anaesthetics increase susceptibility of untransformed cells to agglutination by concanavalin A. Nature. 1975; 253(5492):552-4. DOI: 10.1038/253552a0. View

Nicolson G . Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy. Biochim Biophys Acta. 1976; 458(1):1-72. DOI: 10.1016/0304-419x(76)90014-7. View

Schlessinger J, Koppel D, Axelrod D, Jacobson K, Webb W, Elson E . Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci U S A. 1976; 73(7):2409-13. PMC: 430587. DOI: 10.1073/pnas.73.7.2409. View

Schlessinger J, Axelrod D, Koppel D, Webb W, Elson E . Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science. 1977; 195(4275):307-9. DOI: 10.1126/science.556653. View

Willingham M, Yamada K, Yamada S, Pouyssegur J, Pastan I . Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts. Cell. 1977; 10(3):375-80. DOI: 10.1016/0092-8674(77)90024-1. View

Ben-Bassat H, Polliak A, Rosenbaum S, Naparstek E, Shouval D, Inbar M . Fluidity of membrane lipids and lateral mobility of concanavalin A receptors in the cell surface of normal lymphocytes and lymphocytes from patients with malignant lymphomas and leukemias. Cancer Res. 1977; 37(5):1307-12. View

Bourguignon L, Singer S . Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands. Proc Natl Acad Sci U S A. 1977; 74(11):5031-5. PMC: 432092. DOI: 10.1073/pnas.74.11.5031. View

Mandel G, Clark W . Functional properties of EL-4 tumor cells with lipid-altered membranes. J Immunol. 1978; 120(5):1637-43. View

Braun J, Fujiwara K, Pollard T, UNANUE E . Two distinct mechanisms for redistribution of lymphocyte surface macromolecules. I. Relationship to cytoplasmic myosin. J Cell Biol. 1978; 79(2 Pt 1):409-18. PMC: 2110255. DOI: 10.1083/jcb.79.2.409. View

10.

Zucker-Franklin D, Liebes L, Silber R . Differences in the behavior of the membrane and membrane-associated filamentous structures in normal and chronic lymphocytic leukemia (CLL) lymphocytes. J Immunol. 1979; 122(1):97-107. View

11.

Woda B, FELDMAN J . Density of surface immunoglobulin and capping on rat B lymphocytes. I. Changes with aging. J Exp Med. 1979; 149(2):416-23. PMC: 2184810. DOI: 10.1084/jem.149.2.416. View

12.

Geiduschek J, Singer S . Molecular changes in the membranes of mouse erythroid cells accompanying differentiation. Cell. 1979; 16(1):149-63. DOI: 10.1016/0092-8674(79)90196-x. View

13.

Woda B, Yguerabide J, FELDMAN J . Mobility and density of AgB, "Ia", and Fc receptors on the surface of lymphocytes from young and old rats. J Immunol. 1979; 123(5):2161-7. View

14.

Dragsten P, Henkart P, Blumenthal R, Weinstein J, Schlessinger J . Lateral diffusion of surface immunoglobulin, Thy-1 antigen, and a lipid probe in lymphocyte plasma membranes. Proc Natl Acad Sci U S A. 1979; 76(10):5163-7. PMC: 413100. DOI: 10.1073/pnas.76.10.5163. View

15.

Woda B, Yguerabide J, FELDMAN J . The effect of local anesthetics on the lateral mobility of lymphocyte membrane proteins. Exp Cell Res. 1980; 126(2):327-31. DOI: 10.1016/0014-4827(80)90271-2. View

16.

Eldridge C, Elson E, Webb W . Fluorescence photobleaching recovery measurements of surface lateral mobilities on normal and SV40-transformed mouse fibroblasts. Biochemistry. 1980; 19(10):2075-9. DOI: 10.1021/bi00551a011. View

17.

Golan D, Veatch W . Lateral mobility of band 3 in the human erythrocyte membrane studied by fluorescence photobleaching recovery: evidence for control by cytoskeletal interactions. Proc Natl Acad Sci U S A. 1980; 77(5):2537-41. PMC: 349436. DOI: 10.1073/pnas.77.5.2537. View

18.

Walter R, BERLIN R, Pfeiffer J, Oliver J . Polarization of endocytosis and receptor topography on cultured macrophages. J Cell Biol. 1980; 86(1):199-211. PMC: 2110654. DOI: 10.1083/jcb.86.1.199. View