Alpha 2.0
Login Register
» Articles » PMID: 6968334

Growth of Factor-dependent Hemopoietic Precursor Cell Lines

Overview
Journal J Exp Med
Specialties Allergy & Immunology
General Medicine
Date 1980 Oct 1
PMID 6968334
Citations 166
Authors
T M Dexter
J Garland
D Scott
E Scolnick
D Metcalf
Affiliations
Soon will be listed here.
Abstract

Cell lines have been produced from long-term cultures of mouse bone marrow that require a factor, present in WEHI-3 conditioned medium (CM) or in spleen CM, for their sustained growth. The cell lines were obtained from nonvirus-treated cultures, are nonleukemic, maintain a normal karyotype, and form colonies showing granulocyte maturation when plated in soft agar. Granulocyte/macrophage (GM) colony-stimulating factor is not the inductive moiety involved in the maintenance of proliferation of these cells. It is suggested that the cell lines represent a self-renewing population of cells ancestral to GM colony-forming cells, which may be responding to a hitherto unrecognized regulator.

Citing Articles

A robust approach for the generation of functional hematopoietic progenitor cell lines to model leukemic transformation.

Doma E, Mayer I, Brandstoetter T, Maurer B, Grausenburger R, Menzl I Blood Adv. 2021; 5(1):39-53.

PMID: 33570624 PMC: 7805318. DOI: 10.1182/bloodadvances.2020003022.

The structure-function relationship of oncogenic LMTK3.

Ditsiou A, Cilibrasi C, Simigdala N, Papakyriakou A, Milton-Harris L, Vella V Sci Adv. 2020; 6(46).

PMID: 33188023 PMC: 7673765. DOI: 10.1126/sciadv.abc3099.

In the absence of apoptosis, myeloid cells arrest when deprived of growth factor, but remain viable by consuming extracellular glucose.

Dong L, Reljic B, Cheung J, Ng E, Lindqvist L, Elefanty A Cell Death Differ. 2019; 26(10):2074-2085.

PMID: 30770875 PMC: 6748108. DOI: 10.1038/s41418-019-0275-z.

Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival.

Kizilors A, Pickard M, Schulte C, Yacqub-Usman K, McCarthy N, Gan S Biosci Rep. 2017; 37(4).

PMID: 28754805 PMC: 5634340. DOI: 10.1042/BSR20170843.

RUNX1 cooperates with FLT3-ITD to induce leukemia.

Behrens K, Maul K, Tekin N, Kriebitzsch N, Indenbirken D, Prassolov V J Exp Med. 2017; 214(3):737-752.

PMID: 28213513 PMC: 5339673. DOI: 10.1084/jem.20160927.

References
1.
Williams N, Jackson H, Sheridan A, Murphy Jr M, Elste A, Moore M . Regulation of megakaryopoiesis in long-term murine bone marrow cultures. Blood. 1978; 51(2):245-55. View
2.
Williams N, Eger R, Moore M, MENDELSOHN N . Differentiation of mouse bone marrow precursor cells into neutrophil granulocytes by an activity separation from WEHI-3 cell-conditioned medium. Differentiation. 1978; 11(1):59-63. DOI: 10.1111/j.1432-0436.1978.tb00970.x. View
3.
Greenberger J, Newburger P, Karpas A, MOLONEY W . Constitutive and inducible granulocyte-macrophage functions in mouse, rat, and human myeloid leukemia-derived continuous tissue culture lines. Cancer Res. 1978; 38(10):3340-8. View
4.
Greenberger J . Sensitivity of corticosteroid-dependent insulin-resistant lipogenesis in marrow preadipocytes of obese-diabetic (db/db) mice. Nature. 1978; 275(5682):752-4. DOI: 10.1038/275752a0. View
5.
Metcalf D, Johnson G, Mandel T . Colony formation in agar by multipotential hemopoietic cells. J Cell Physiol. 1979; 98(2):401-20. DOI: 10.1002/jcp.1040980216. View