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Isolation of Genes Controlling Apoptosis Through Their Effects on Cell Survival

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Date 2007 Mar 21
PMID 17372619
Citations 15
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Abstract

The identification of the most suitable molecular targets for gene and drug therapy is the crucial first step in the development of new disease treatments. The rational identification of such targets depends on a detailed understanding of the pathological changes occuring at the molecular level. We have applied forward genetics approaches to the identification of the critical genes involved in the control of apoptosis in mammalian cells, since defective control of apoptosis underlies many diseases, including cancer and neurodegenerative diseases. We have identified two groups of genes by their effects on cell survival using retroviral cDNA functional expression cloning and retroviral insertional mutagenesis. The identification of these novel genes opens up new areas for apoptosis research and subsequently for the development of new gene and drug therapies.

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References
1.
Liliental J, Chang D . Rack1, a receptor for activated protein kinase C, interacts with integrin beta subunit. J Biol Chem. 1998; 273(4):2379-83. DOI: 10.1074/jbc.273.4.2379. View

2.
Jundt F, Anagnostopoulos I, Forster R, Mathas S, Stein H, Dorken B . Activated Notch1 signaling promotes tumor cell proliferation and survival in Hodgkin and anaplastic large cell lymphoma. Blood. 2002; 99(9):3398-403. DOI: 10.1182/blood.v99.9.3398. View

3.
Dexter T, Garland J, Scott D, Scolnick E, Metcalf D . Growth of factor-dependent hemopoietic precursor cell lines. J Exp Med. 1980; 152(4):1036-47. PMC: 2185980. DOI: 10.1084/jem.152.4.1036. View

4.
KERR J, Winterford C, Harmon B . Apoptosis. Its significance in cancer and cancer therapy. Cancer. 1994; 73(8):2013-26. DOI: 10.1002/1097-0142(19940415)73:8<2013::aid-cncr2820730802>3.0.co;2-j. View

5.
Anderson C, Williams G . Apoptosis gene hunting using retroviral expression cloning: identification of vacuolar ATPase subunit E. ScientificWorldJournal. 2003; 3:51-8. PMC: 5974873. DOI: 10.1100/tsw.2003.07. View