Adrenal Opioid Proteins of 8600 and 12,600 Daltons: Intermediates in Proenkephalin Processing

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Mar 1

PMID 6952256

Citations 3

Authors

B N Jones

J E Shively

D L Kilpatrick

A S Stern

R V Lewis

K Kojima

S UDENFRIEND

Affiliations

Soon will be listed here.

Abstract

[Met]Enkephalin-containing proteins of 8600 and 12,600 daltons have been isolated from acid extracts of bovine adrenal medulla and purified to homogeneity, and their sequences have been determined by a combination of automated Edman degradation, tryptic mapping, and enzymatic time-course hydrolysis. The 8600-dalton protein contains one copy of the [Met]enkephalin sequence at the COOH terminus and the 12,600-dalton protein contains three copies of [Met]enkephalin, of which two are internal and the third is at the COOH terminus. They possess identical NH2-terminal amino acid sequences, suggesting that the 8600-dalton protein is derived from the 12,600-dalton protein by intracellular proteolytic processing. This is supported by results from tryptic maps of both proteins. Furthermore, chemical analysis of the tryptic peptides obtained from the 12,600-dalton protein indicates that it also contains the amino acid sequence that corresponds to a previously characterized enkephalin-containing polypeptide of 3800 daltons (peptide F) [Jones et al. (1980) Arch. Biochem. Biophys. 204, 392-395]. All three polypeptides appear to be intermediates in posttranslational processing of a still larger polyenkephalin precursor molecule, proenkephalin, and part of a biosynthetic pathway leading to smaller enkephalin-containing polypeptides and free enkephalins.

Citing Articles

An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.

Kilpatrick D, Jones B, Lewis R, Stern A, Kojima K, Shively J Proc Natl Acad Sci U S A. 1982; 79(9):3057-61.

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Cloning and sequencing of human intestinal alkaline phosphatase cDNA.

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