Purification to Homogeneity of Camel Pituitary Pro-opiocortin, the Common Precursor of Opioid Peptides and Corticotropin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Apr 1

PMID 221906

Citations 12

Authors

S Kimura

R V Lewis

L D Gerber

L Brink

M Rubinstein

S Stein

S UDENFRIEND

Affiliations

Soon will be listed here.

Abstract

Pro-opiocortin was purified from camel pituitaries by procedures including high-performance liquid chromatography. The precursor relationship of the pure protein to the opioid peptides and to corticotropin was confirmed. Partial chemical analysis consisting of amino acid analysis and tryptic peptide mapping was carried out with the aid of sensitive fluorescence detection.

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References

Gerber L, Stein S, Rubinstein M, Wideman J, UDENFRIEND S . Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site. Brain Res. 1978; 151(1):117-26. DOI: 10.1016/0006-8993(78)90954-x. View

Nakanishi S, Inoue A, Kita T, Numa S, Chang A, Cohen S . Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor. Proc Natl Acad Sci U S A. 1978; 75(12):6021-5. PMC: 393109. DOI: 10.1073/pnas.75.12.6021. View

Li C, Chung D, Yamashiro D, Lee C . Isolation, characterization, and synthesis of a corticotropin-inhibiting peptide from human pituitary glands. Proc Natl Acad Sci U S A. 1978; 75(9):4306-9. PMC: 336102. DOI: 10.1073/pnas.75.9.4306. View

Roberts J, Phillips M, Rosa P, Herbert E . Steps involved in the processing of common precursor forms of adrenocorticotropin and endorphin in cultures of mouse pituitary cells. Biochemistry. 1978; 17(17):3609-18. DOI: 10.1021/bi00610a030. View

Roberts J, Herbert E . Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule. Proc Natl Acad Sci U S A. 1977; 74(11):4826-30. PMC: 432048. DOI: 10.1073/pnas.74.11.4826. View

Rubinstein M, Stein S, UDENFRIEND S . Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin. Proc Natl Acad Sci U S A. 1978; 75(2):669-71. PMC: 411317. DOI: 10.1073/pnas.75.2.669. View

Eipper B, Mains R . Analysis of the common precursor to corticotropin and endorphin. J Biol Chem. 1978; 253(16):5732-44. View

Rubinstein M, Stein S, Gerber L, UDENFRIEND S . Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin. Proc Natl Acad Sci U S A. 1977; 74(7):3052-5. PMC: 431409. DOI: 10.1073/pnas.74.7.3052. View

Mains R, Eipper B, Ling N . Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977; 74(7):3014-8. PMC: 431388. DOI: 10.1073/pnas.74.7.3014. View

10.

Rubinstein M, Stein S, UDENFRIEND S . Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin. Proc Natl Acad Sci U S A. 1977; 74(11):4969-72. PMC: 432079. DOI: 10.1073/pnas.74.11.4969. View

11.

Ling N, Burgus R, Guillemin R . Isolation, primary structure, and synthesis of alpha-endorphin and gamma-endorphin, two peptides of hypothalamic-hypophysial origin with morphinomimetic activity. Proc Natl Acad Sci U S A. 1976; 73(11):3942-6. PMC: 431275. DOI: 10.1073/pnas.73.11.3942. View

12.

Scott A, Ratcliffe J, Rees L, LANDON J, Bennett H, Lowry P . Pituitary peptide. Nat New Biol. 1973; 244(133):65-7. DOI: 10.1038/newbio244065a0. View

13.

Stein S, Bohlen P, Stone J, Dairman W, UDENFRIEND S . Amino acid analysis with fluorescamine at the picomole level. Arch Biochem Biophys. 1973; 155(1):202-12. DOI: 10.1016/s0003-9861(73)80022-0. View

14.

Li C, Chung D . Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands. Proc Natl Acad Sci U S A. 1976; 73(4):1145-8. PMC: 430217. DOI: 10.1073/pnas.73.4.1145. View

15.

Bohlen P, Stein S, Stone J, UDENFRIEND S . Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975; 67(2):438-45. DOI: 10.1016/0003-2697(75)90316-4. View

16.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View