Differing Effects of Monoclonal Anti-hapten Antibodies on Humoral Responses to Soluble or Particulate Antigens

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1984 May 1

PMID 6715016

Citations 21

Authors

G G Klaus

Affiliations

Soon will be listed here.

Abstract

This study compares the effects of passive administration of monoclonal anti-hapten (DNP) antibodies on primary plaque-forming cell (PFC) responses in mice to either soluble (DNP-keyhole limpet haemocyanin [KLH] ) or particulate (TNP-erythrocyte) antigens. IgM, IgG1, IgG2a and IgG2b antibodies at doses up to 500 micrograms induced at best a modest suppression of the IgM response, and reproducibly enhanced the IgG response to DNP-KLH by up to 30-fold. In contrast, with the particulate antigen only the IgM antibody enhanced IgG PFC; IgG2 antibodies, and one out of two IgG1 antibodies caused marked suppression of the primary response to TNP-RBC. This required antibody with an intact Fc portion. The enhancement of IgG responses to soluble antigen presumably reflects rapid B cell priming by immune complexes trapped by follicular dendritic cells in lymphoid follicles, in agreement with earlier data. These results indicate that the nature of the antigen can markedly influence the immunoregulatory effects of antibodies on humoral responses.

Citing Articles

Influenza virus antibodies inhibit antigen-specific B cell responses in mice.

Goodwin E, Gibbs J, Yewdell J, Eisenlohr L, Hensley S J Virol. 2024; 98(9):e0076624.

PMID: 39194245 PMC: 11406888. DOI: 10.1128/jvi.00766-24.

Antibody feedback regulation.

Heyman B Immunol Rev. 2024; 328(1):126-142.

PMID: 39180190 PMC: 11659925. DOI: 10.1111/imr.13377.

Influenza virus antibodies inhibit antigen-specific B cell responses in mice.

Goodwin E, Gibbs J, Yewdell J, Eisenlohr L, Hensley S bioRxiv. 2024; .

PMID: 38659819 PMC: 11042189. DOI: 10.1101/2024.04.12.589218.

Complement C3 and marginal zone B cells promote IgG-mediated enhancement of RBC alloimmunization in mice.

Jash A, Pridmore T, Collins J, Hay A, Hudson K, Luckey C J Clin Invest. 2024; 134(8).

PMID: 38618959 PMC: 11014669. DOI: 10.1172/JCI167665.

IgG Subclass Determines Suppression Versus Enhancement of Humoral Alloimmunity to Kell RBC Antigens in Mice.

Shinde P, Howie H, Stegmann T, Hay A, Waterman H, Szittner Z Front Immunol. 2020; 11:1516.

PMID: 32765523 PMC: 7378678. DOI: 10.3389/fimmu.2020.01516.

References

Wigzell H . Antibody synthesis at the cellular level. Antibody-induced suppression of 7S antibody synthesis. J Exp Med. 1966; 124(5):953-69. PMC: 2138261. DOI: 10.1084/jem.124.5.953. View

Henry C, Jerne N . Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968; 128(1):133-52. PMC: 2138503. DOI: 10.1084/jem.128.1.133. View

UHR J, Moller G . Regulatory effect of antibody on the immune response. Adv Immunol. 1968; 8:81-127. DOI: 10.1016/s0065-2776(08)60465-4. View

Dennert G . The mechanism of antibody-induced stimulation and inhibition of the immune response. J Immunol. 1971; 106(4):951-5. View

Murgita R, Vas S . Specific antibody-mediated effect on the immune response. Suppression and augmentation of the primary immune response in mice by different classes of antibodies. Immunology. 1972; 22(3):319-31. PMC: 1407808. View

Schrader J . Regulation of the immune response by IgM antibody: a paradoxical suppression of the in vitro primary immune response to sheep erythrocytes by passive IgM. Aust J Exp Biol Med Sci. 1973; 51(3):333-46. DOI: 10.1038/icb.1973.32. View

StC Sinclair N . Mechanisms of immunoregulation. Immunoregulation by antibody and antigen-antibody complexes. Transplant Proc. 1978; 10(2):349-53. View

Klaus G . Generation of memory cells. III. Antibody class requirements for the generation of B-memory cells by antigen--antibody complexes. Immunology. 1979; 37(2):345-51. PMC: 1457494. View

Ey P, Prowse S, JENKIN C . Complement-fixing IgG1 constitutes a new subclass of mouse IgG. Nature. 1979; 281(5731):492-3. DOI: 10.1038/281492a0. View

10.

Klaus G, Pepys M, Kitajima K, Askonas B . Activation of mouse complement by different classes of mouse antibody. Immunology. 1979; 38(4):687-95. PMC: 1457877. View

11.

Klaus G, Humphrey J, Kunkl A, Dongworth D . The follicular dendritic cell: its role in antigen presentation in the generation of immunological memory. Immunol Rev. 1980; 53:3-28. DOI: 10.1111/j.1600-065x.1980.tb01038.x. View

12.

Kunkl A, Klaus G . The generation of memory cells. V. Preferential priming of IgG1 B memory cells by immunization with antigen IgG2 antibody complexes. Immunology. 1981; 44(1):163-8. PMC: 1555111. View

13.

Coutinho A, Forni L . Antigen-specific and -non-specific regulatory influences of antibody in immune responses are determined by variable region properties. Ann Immunol (Paris). 1981; 132C(2):131-44. DOI: 10.1016/0769-2625(81)90022-2. View

14.

Neuberger M, Rajewsky K . Activation of mouse complement by monoclonal mouse antibodies. Eur J Immunol. 1981; 11(12):1012-6. DOI: 10.1002/eji.1830111212. View

15.

Heyman B, Andrighetto G, Wigzell H . Antigen-dependent IgM-mediated enhancement of the sheep erythrocyte response in mice. Evidence for induction of B cells with specificities other than that of the injected antibodies. J Exp Med. 1982; 155(4):994-1009. PMC: 2186654. DOI: 10.1084/jem.155.4.994. View

16.

Powell R, Hutchings P, Cooke A, Lydyard P . Antibody mediated regulation of immune responses. I. Enhancement of specific antibody responses through IgM antibodies. Immunol Lett. 1982; 4(5):253-8. DOI: 10.1016/0165-2478(82)90047-5. View

17.

Bruggemann M, Rajewsky K . Regulation of the antibody response against hapten-coupled erythrocytes by monoclonal antihapten antibodies of various isotypes. Cell Immunol. 1982; 71(2):365-73. DOI: 10.1016/0008-8749(82)90270-2. View

18.

OVARY Z . Recent insight into class-specific properties of murine immunoglobulins obtained with the help of monoclonal antibodies. Int Arch Allergy Appl Immunol. 1982; 69(4):385-92. DOI: 10.1159/000233205. View

19.

Collisson E, Anderson B, Ronnholm M, Lamon E . Potentiation of antibody responses by specific IgM: specificity and thymus dependency. Cell Immunol. 1983; 79(1):44-55. DOI: 10.1016/0008-8749(83)90049-7. View

20.

Klaus G . Follicular trapping of hapten-erythrocyte-antibody complexes in mouse spleen. Immunology. 1984; 52(1):107-16. PMC: 1454585. View