Influenza Virus Antibodies Inhibit Antigen-specific B Cell Responses in Mice

Overview

Journal bioRxiv

Date 2024 Apr 25

PMID 38659819

Authors

Eileen Goodwin

James S Gibbs

Jonathan W Yewdell

Laurence C Eisenlohr

Scott E Hensley

Affiliations

Soon will be listed here.

Abstract

Antibody responses to influenza vaccines tend to be focused on epitopes encountered during prior influenza exposures, with little production of responses to novel epitopes. To examine the contribution of circulating antibody to this phenomenon, we passively transferred a hemagglutinin (HA)-specific monoclonal antibody (mAb) into mice before immunizing with whole inactivated virions. The HA mAb inhibited HA-specific antibodies, plasmablasts, germinal center B cells, and memory B cells, while responses to a second antigen in the vaccine, neuraminidase (NA), were uninhibited. The HA mAb potently inhibited antibody responses against epitopes near the HA mAb binding site. The HA mAb also promoted IgG1 class switching, an effect that, unlike the inhibition of HA responses, relied on signaling through Fc-gamma receptors. These studies suggest that circulating antibodies inhibit B cell responses in an antigen-specific manner, which likely contributes to differences in antibody specificities elicited during primary and secondary influenza virus exposures.

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