Cross-reactivity of Human and a Putative Pig IgD. Pig IgD-like Molecules As Serum and Lymphocyte Components

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 1983 Jan 1

PMID 6667917

Citations 3

Authors

J Zikan

P Sima

L Tuckova

Affiliations

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Abstract

Proteins of pig serum and splenocytes which were isolated on immobilized guinea pig antibodies against human IgD were characterized by SDS-PAGE. In both cases the main isolated components possessed several properties nearly identical with those of human IgD: molar mass of the components and their chains, and susceptibility of the heavy chains, light chains, the chymotryptic peptide maps of the chains, and susceptibility of the heavy chains, the chymotryptic peptide maps of the chains, and susceptibility of the heavy chains in the native molecule to trypsin digestion. These IgD-like components not adsorbed on immobilized normal guinea-pig IgG. We suggest that the components of pig serum and splenocytes cross-reacting with highly specific antibodies against human IgD represent candidates for pig IgD. The percentage of the IgD-like molecules on the surface of pig splenocytes labeled with the antibody against human IgD was similar to the percentage of splenocytes bearing all immunoglobulins. Therefore, we suggest that IgD-like molecules were occurring on the surface of cells mostly together with molecules of another immunoglobulin class.

Citing Articles

The appearance of an IgD-like molecule on pig lymphocytes during ontogeny.

Trebichavsky I, Zikan J, Travnicek J Folia Microbiol (Praha). 1983; 28(6):484-8.

PMID: 6667918 DOI: 10.1007/BF02879687.

Immunoglobulin D in rat serum, saliva and milk.

Steele M, Leslie G Immunology. 1985; 55(4):571-7.

PMID: 4018841 PMC: 1453765.

IgD antibodies to DNP are specifically induced in ascitic fluid after intraperitoneal immunization.

Steele M, Leslie G Immunology. 1988; 64(3):391-5.

PMID: 3137154 PMC: 1385048.

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