Peptide Variability Exists Within Alpha and Beta Subunits of the T Cell Receptor for Antigen

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1983 Oct 1

PMID 6604785

Citations 26

Authors

O Acuto

S C Meuer

J C Hodgdon

S F Schlossman

E L Reinherz

Affiliations

Soon will be listed here.

Abstract

The T cell receptor for antigen (Ti) has recently been identified as a 90-kdalton T3-associated clonotypic structure composed of one 49-51-kdalton alpha and one 43-kdalton beta subunit, which are disulfide linked. Here, Ti molecules from two alloreactive CTL clones derived from the same donor but of differing specificities (CT8III and CT4II) are directly compared following isolation with anticlonotypic monoclonal antibodies. Isoelectric focusing shows that the alpha subunits (pI 4.4-4.7) are more acidic than the beta subunits (pI 6.0-6.2) but that each glycoprotein species is distinctive. More importantly, two-dimensional peptide maps of 125I-labeled surface receptors indicate that the beta chains of Ti1 and Ti2 appear unique and share only two peptides in common. In contrast, peptide maps of Ti1 and Ti2 alpha chains are more related although not identical. These results suggest that the human T cell receptor is composed of constant as well as variable regions and that at least one of the latter is located within the beta subunit.

Citing Articles

αβ TCR-mediated recognition: relevance to tumor-antigen discovery and cancer immunotherapy.

Reinherz E Cancer Immunol Res. 2015; 3(4):305-12.

PMID: 25847967 PMC: 4391277. DOI: 10.1158/2326-6066.CIR-15-0042.

Revisiting the Discovery of the αβ TCR Complex and Its Co-Receptors.

Reinherz E Front Immunol. 2014; 5:583.

PMID: 25484883 PMC: 4240158. DOI: 10.3389/fimmu.2014.00583.

Toward a molecular understanding of adaptive immunity: a chronology - part II.

Smith K Front Immunol. 2012; 3:364.

PMID: 23230441 PMC: 3515962. DOI: 10.3389/fimmu.2012.00364.

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.

Wang J, Reinherz E Immunol Rev. 2012; 250(1):102-19.

PMID: 23046125 PMC: 3694212. DOI: 10.1111/j.1600-065X.2012.01161.x.

Molecular T cell biology -- basic and translational challenges in the twenty-first century.

Reinherz E, Acuto O Front Immunol. 2012; 2:3.

PMID: 22566794 PMC: 3342379. DOI: 10.3389/fimmu.2011.00003.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

Brack C, Hirama M, Tonegawa S . A complete immunoglobulin gene is created by somatic recombination. Cell. 1978; 15(1):1-14. DOI: 10.1016/0092-8674(78)90078-8. View

Seidman J, Max E, Leder P . A kappa-immunoglobulin gene is formed by site-specific recombination without further somatic mutation. Nature. 1979; 280(5721):370-5. DOI: 10.1038/280370a0. View

Omary M, Trowbridge I . Disposition of T200 glycoprotein in the plasma membrane of a murine lymphoma cell line. J Biol Chem. 1980; 255(4):1662-9. View

Early P, Huang H, Davis M, Calame K, Hood L . An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH. Cell. 1980; 19(4):981-92. DOI: 10.1016/0092-8674(80)90089-6. View

Corte G, Damiani G, Calabi F, Fabbi M, Bargellesi A . Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. Proc Natl Acad Sci U S A. 1981; 78(1):534-8. PMC: 319088. DOI: 10.1073/pnas.78.1.534. View

Meuer S, Schlossman S, Reinherz E . Clonal analysis of human cytotoxic T lymphocytes: T4+ and T8+ effector T cells recognize products of different major histocompatibility complex regions. Proc Natl Acad Sci U S A. 1982; 79(14):4395-9. PMC: 346678. DOI: 10.1073/pnas.79.14.4395. View

Meuer S, Fitzgerald K, Hussey R, Hodgdon J, Schlossman S, Reinherz E . Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex. J Exp Med. 1983; 157(2):705-19. PMC: 2186929. DOI: 10.1084/jem.157.2.705. View

10.

Haskins K, Kubo R, White J, Pigeon M, Kappler J, Marrack P . The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med. 1983; 157(4):1149-69. PMC: 2186983. DOI: 10.1084/jem.157.4.1149. View

11.

Meuer S, Acuto O, Hussey R, Hodgdon J, Fitzgerald K, Schlossman S . Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor. Nature. 1983; 303(5920):808-10. DOI: 10.1038/303808a0. View

12.

Reinherz E, Meuer S, Fitzgerald K, Hussey R, Hodgdon J, Acuto O . Comparison of T3-associated 49- and 43-kilodalton cell surface molecules on individual human T-cell clones: evidence for peptide variability in T-cell receptor structures. Proc Natl Acad Sci U S A. 1983; 80(13):4104-8. PMC: 394209. DOI: 10.1073/pnas.80.13.4104. View