Effect of Pimozide on the Cytology of the Eel Pituitary. II. MSH-secreting Cells

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1978 May 29

PMID 657240

Citations 10

Authors

M Olivereau

Affiliations

Soon will be listed here.

Abstract

Pimozide, a specific blocker of dopaminergic receptors, was injected for 4 or 9 days in freshwater (FW) eels or eels acclimated to sea water (SW) for 10 or 30 days. The daily dose was 100 or 200 microgram/100 g. Melanophore index values increase in FW and in 1 month-SW injected eels. All the treated fish react by a total or subtotal degranulation of the lead-hematoxylin positive cells in the pars intermedia. These cells were previously identified as alpha-MSH-secreting cells. The MSH cell nuclear area is significantly increased, nucleoli are larger and the endoplasmic reticulum more developed. The intensity of the response is similar in FW and SW eels, but it does not increase with the higher dose. The rapid release of pituitary alpha-MSH is also visualized by immunofluorescence and immunoenzymologic techniques. No effect on the second cell type of the pars intermedia (PAS-positive cell) is detected. The amount of neurosecretory material is often reduced in the neurohypophysis. These results suggest that the hypothalamic inhibitory control of MSH release and synthesis is mediated through dopaminergic fibers in the eel, but other factors cannot be ignored in this regulation.

Citing Articles

Ultrastructural changes in the calcium-sensitive (PAS-positive) cells of the pars intermedia of eels kept in deionized water and in normal and concentrated sea water.

Olivereau M, CHAMBOLLE P, Dubourg P Cell Tissue Res. 1981; 219(1):9-26.

PMID: 7285100 DOI: 10.1007/BF00210015.

Effects of pimozide on the ultrastructure of the pars intermedia in the rat.

Santolaya R, Ciocca D Cell Tissue Res. 1981; 217(2):397-403.

PMID: 7237535 DOI: 10.1007/BF00233589.

Responses of MSH and prolactin cells to 5-hydroxytryptophan (5-HTP) in amphibians and teleosts.

Olivereau M, Olivereau J, Aimar C Cell Tissue Res. 1980; 207(3):377-85.

PMID: 6967354 DOI: 10.1007/BF00224614.

Ultrastructural identification of catecholaminergic fibers in the goldfish pituitary. A high-resolution radioautographic study after in vitro 3H-dopamine administration.

Kah O, Dubourg P, CHAMBOLLE P, Calas A Cell Tissue Res. 1984; 238(3):621-6.

PMID: 6525624 DOI: 10.1007/BF00219880.

The dopaminergic innervation of the goldfish pituitary. An immunocytochemical study at the electron-microscope level using antibodies against dopamine.

Kah O, Dubourg P, Onteniente B, Geffard M, Calas A Cell Tissue Res. 1986; 244(3):577-82.

PMID: 3719676 DOI: 10.1007/BF00212536.

References

Olivereau M, BALL J . Histological study of functional ectopic pituitary transplants in a teleost fish (Poecilia formosa). Proc R Soc Lond B Biol Sci. 1966; 164(994):106-29. DOI: 10.1098/rspb.1966.0016. View

TRYGSTAD O, Foss I . Congenital generalized lipodystrophy and experimental lipoatrophic diabetes in rabbits treated successfully with fenfluramine. Acta Endocrinol (Copenh). 1977; 85(2):436-48. DOI: 10.1530/acta.0.0850436. View

Olivereau M . [Activity of the pars intermedia of the autotransplanted pituitary gland in the European eel]. Z Zellforsch Mikrosk Anat. 1969; 98(1):74-87. View

Corbin A, UPTON G, MABRY C, HOLLINGSWORTH D . Diencephalic involvement in generalized lipodystrophy: rationale and treatment with the neuroleptic agent, pimozide. Acta Endocrinol (Copenh). 1974; 77(2):209-20. DOI: 10.1530/acta.0.0770209. View

Olivereau M . [Elaboration of intermedin by the cells stained with lead hematoxyline in the pars intermedia of the eel: new proofs and hypothalamic control]. C R Acad Hebd Seances Acad Sci D. 1971; 272(1):102-5. View

Rychter J, Stepien H . Effects of pimozide and bromocriptine on the proliferation of rat pituitary pars intermedia cells. J Endocrinol. 1977; 75(3):443-4. DOI: 10.1677/joe.0.0750443. View

Olivereau M, Bugnon C, Fellmann D . [Cyto-immunologic location of alpha-MSH and ACTH in the lead-hematoxylin (HPb)-stainable pituitary cells, in the eel]. C R Acad Hebd Seances Acad Sci D. 1976; 283(11 D):1321-3. View

Kastin A, Schally A, Viosca S . Inhibition of MSH release in frogs by direct application of L-proplyl-L-leucylglycinamide to the pituitary. Proc Soc Exp Biol Med. 1971; 137(4):1437-9. DOI: 10.3181/00379727-137-35805. View

FOLLENIUS E . [Integration of dopamine in aminergic endings of the meta-adenohypophysis of the sticklebach (Gasterosteus aculeatus L.)]. C R Acad Hebd Seances Acad Sci D. 1971; 273(12):1039-40. View

10.

Fremberg M, MEURLING P . Catecholamine fluorescence in the pituitary of the eel, Anguilla anguilla, with special reference to its variation during background adaptation. Cell Tissue Res. 1975; 157(1):53-72. DOI: 10.1007/BF00223230. View

11.

Goos H, Terlou M . Hypothalamic control of MSH secretion in lower vertebrates. Front Horm Res. 1977; 4:51-62. DOI: 10.1159/000400349. View

12.

Sage M . Control of prolactin release and its role in color change in the teleost Gillichthys mirabilis. J Exp Zool. 1970; 173(1):121-7. DOI: 10.1002/jez.1401730109. View

13.

Morgan C, Hadley M . Ergot alkaloid inhibition of melanophore stimulating hormone secretion. Neuroendocrinology. 1976; 21(1):10-9. DOI: 10.1159/000122605. View

14.

Janssen P, NIEMEGEERS C, SCHELLEKENS K, Dresse A, Lenaerts F, PINCHARD A . Pimozide, a chemically novel, highly potent and orally long-acting neuroleptic drug. I. The comparative pharmacology of pimozide, haloperidol, and chlorpromazine. Arzneimittelforschung. 1968; 18(3):261-79. View

15.

Olivereau M . Effect of pimozide on the cytology of the eel pituitary. I. Prolactin-secreting cells. Cell Tissue Res. 1978; 189(2):219-30. DOI: 10.1007/BF00209271. View

16.

Olivereau M . [Neuro-intermediate lobe and osmoregulation: comparison of the European eel and the cultured Japanese eel during a transfer from fresh water to sea water]. Z Zellforsch Mikrosk Anat. 1969; 99(3):389-410. View

17.

Kah O, CHAMBOLLE P, Olivereau M . [Aminergic innervation of the hypothalamo-hypophyseal system in Gambusia sp. (Teleost, Poecilidae) studied by two fluorescence techniques]. C R Acad Hebd Seances Acad Sci D. 1978; 286(9):705-8. View

18.

Olivereau M . [Action of reserpine in eels. II. Effect on pigmentation and pars intermedia. Comparison with the effect of a black background adaptation]. Z Anat Entwicklungsgesch. 1972; 137(1):30-46. View

19.

Thornton V, Howe C . The effect of change of background colour on the ultrastructure of the pars intermedia of the pituitary of the eel (Anguilla anguilla). Cell Tissue Res. 1974; 151(1):103-15. DOI: 10.1007/BF00222038. View

20.

Baker B . The cellular source of melanocyte-stimulating hormone in Anguilla pituitary. Gen Comp Endocrinol. 1972; 19(3):515-21. DOI: 10.1016/0016-6480(72)90250-x. View