Ultrastructural Changes in the Calcium-sensitive (PAS-positive) Cells of the Pars Intermedia of Eels Kept in Deionized Water and in Normal and Concentrated Sea Water

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1981 Jan 1

PMID 7285100

Citations 8

Authors

M Olivereau

P CHAMBOLLE

P Dubourg

Affiliations

Soon will be listed here.

Abstract

The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparatus, a distinct rough endoplasmic reticulum, few secretory granules, some microtubules and an extended area of contact with the basal lamina that separates nervous and glandular tissues. Some mitosing cells were seen. A similar picture was observed in eels kept in sea water (SW) for 45 days, returned to FW and subsequently to DW for 21 days. In SW (20 and 33%), and particularly in concentrated SW (50, 60 and 63%), the Ca-s cells were inactive. Their granules were significantly smaller than in eels kept in FW, and the area of contact with the basal lamina was greatly reduced. However, signs of granule-release were seen in eels adapted to 50 and 60% SW. Nerve fibers rarely contacted the Ca-s cells and did not synapse with them. The ultrastructural data support the hypothesis that the Ca-s cells of Anguilla, like those of Carassius, are involved in ionic regulation. MSH cells were not greatly affected by the present experiments.

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References

Olivereau M, CHAMBOLLE P . Serotoninergic control of MSH secretion in the eel. Ultrastructural study after 5-hydroxytryptophan treatment. J Physiol (Paris). 1979; 75(1):109-12. View

Batten T, BALL J, Benjamin M . Ultrastructure of the adenohypophysis in the teleost Poecilia latipinna. Cell Tissue Res. 1975; 161(2):239-61. DOI: 10.1007/BF00220372. View

Olivereau M, Aimar C, Olivereau J . PAS-positive cells of the pars intermedia are calcium-sensitive in the goldfish maintained in a hyposmotic milieu. Cell Tissue Res. 1980; 212(1):29-38. DOI: 10.1007/BF00234030. View

Olivereau M . [Reactions observed in eels kept in a medium deprived of electrolytes, especially in the hypothalamo-hypophyseal system]. Z Zellforsch Mikrosk Anat. 1967; 80(2):264-85. View

Olivereau M, CHAMBOLLE P . [Ultrastructure of gonadotropic cells of the normal eel and after estradiol injection]. C R Acad Hebd Seances Acad Sci D. 1978; 287(16):1409-12. View

Benjamin M . A morphometric study of the pituitary cell types in the freshwater stickleback, Gasterosteus aculeatus, form leiurus. Cell Tissue Res. 1974; 152(1):69-92. DOI: 10.1007/BF00224211. View

Dubourg P, CHAMBOLLE P, Olivereau M, Kah O . [Ultrastructure of prolactin cells of Gambusia (teleost fish) in fresh water, deionized water and hypersaline medium]. Gen Comp Endocrinol. 1980; 41(4):554-62. DOI: 10.1016/0016-6480(80)90061-1. View

Kah O, CHAMBOLLE P, Olivereau M, Dubourg P . [Pituitary ultrastructure in Gambusia sp. (teleostean fish) in situ and after long-term graft. 1. Rostral pars distalis]. Gen Comp Endocrinol. 1979; 38(2):253-63. DOI: 10.1016/0016-6480(79)90214-4. View

Benjamin M . The adenohypophysis of the flounder, Pleuronectes flesus, and the minnow, Phoxinus phoxinus. Cell Tissue Res. 1975; 157(3):391-409. DOI: 10.1007/BF00225528. View

10.

Olivereau M . Effect of pimozide on the cytology of the eel pituitary. II. MSH-secreting cells. Cell Tissue Res. 1978; 189(2):231-9. DOI: 10.1007/BF00209272. View

11.

Olivereau M, Aimar C, Olivereau J . Responses of the teleost pituitary (goldfish, eel) to deionized water. Cell Tissue Res. 1980; 208(3):389-404. DOI: 10.1007/BF00233872. View

12.

Abraham M . Ultrastructure of the cell types and of the neurosecretory innervation in the pituitary of Mugil cephalus L. from freshwater, the sea, and a hypersaline lagoon III. The neuro-intermediate lobe. Gen Comp Endocrinol. 1976; 29(4):511-21. DOI: 10.1016/0016-6480(76)90035-6. View

13.

Harrisson F, DEMAL J, Remacle C . [Cytophysiological changes in pituitary gland (pars intermedia) of Carassius auratus L. (Teleostei cyprinidae) during adaptation to hyperosmotic environment]. Arch Anat Microsc Morphol Exp. 1974; 63(4):299-306. View

14.

Leatherland J . Seasonal variation in the structure and ultrastructure of the pituitary gland in the marine form (Trachurus) of the threespine stickleback Gasterosteus aculeatus L. II. Proximal pars distalis and neuro-intermediate lobe. Z Zellforsch Mikrosk Anat. 1970; 104(3):318-36. DOI: 10.1007/BF00335685. View

15.

Olivereau M . [Neuro-intermediate lobe and osmoregulation: comparison of the European eel and the cultured Japanese eel during a transfer from fresh water to sea water]. Z Zellforsch Mikrosk Anat. 1969; 99(3):389-410. View

16.

Olivereau M . [Action of reserpine in eels. II. Effect on pigmentation and pars intermedia. Comparison with the effect of a black background adaptation]. Z Anat Entwicklungsgesch. 1972; 137(1):30-46. View

17.

CHAMBOLLE P, Kah O, Olivereau M, Dubourg P, Olivereau J . [Ultrastructure of the hypophysis in Gambusia sp. (teleostfish) in situ and grafted for a long time. II. Proximal pars distalis]. Gen Comp Endocrinol. 1981; 43(1):85-95. DOI: 10.1016/0016-6480(81)90035-6. View

18.

Thornton V, Howe C . The effect of change of background colour on the ultrastructure of the pars intermedia of the pituitary of the eel (Anguilla anguilla). Cell Tissue Res. 1974; 151(1):103-15. DOI: 10.1007/BF00222038. View

19.

Jasinski A, Kilarski W . Fine structure of pars neuro-intermedia of the loach, Misgurnus fossilis L. Z Zellforsch Mikrosk Anat. 1970; 105(2):259-75. DOI: 10.1007/BF00335476. View

20.

Abraham M, Lotan R . The pituitary of Aphanius dispar (Rüppell) from hypersaline marshes and freshwater. II. Ultrastructure of the rostral pars distalis. Cell Tissue Res. 1977; 179(3):317-30. DOI: 10.1007/BF00221103. View