The Dopaminergic Innervation of the Goldfish Pituitary. An Immunocytochemical Study at the Electron-microscope Level Using Antibodies Against Dopamine

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1986 Jan 1

PMID 3719676

Citations 11

Authors

O Kah

P Dubourg

B Onteniente

M Geffard

A Calas

Affiliations

Soon will be listed here.

Abstract

The dopaminergic innervation of the goldfish pituitary gland was studied by immunocytochemistry at the electron-microscope level using highly specific antibodies against dopamine coupled to bovine serum albumin with glutaraldehyde. A satisfactory preservation of the tissue was achieved after immersion in 5% glutaraldehyde in phosphate buffer containing sodium metabisulfite to prevent oxidation of the endogenous dopamine. The immunocytochemical procedure was performed on Vibratome sections using the preembedding method. Immunoreactivity was restricted to part of the neurosecretory type-B fibers (diameter of the secretory vesicles lower than 100 nm) in which it was found to occupy the whole cytoplasm. Labeled fibers were observed within the neurohypophysis in the different parts of the gland and in the adenohypophyseal tissue where immunoreactive profiles were detected in close apposition to the different cell types. These data are in agreement with previous results obtained by means of radioautography and further support a role for dopamine in the neuroendocrine regulation of pituitary functions in teleosts.

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References

Olivereau M . Dopamine, prolactin control, and osmoregulation in eels. Gen Comp Endocrinol. 1975; 26(4):550-61. DOI: 10.1016/0016-6480(75)90178-1. View

Chang J, Marchant T, Cook A, Nahorniak C, Peter R . Influences of catecholamines on growth hormone release in female goldfish, Carassius auratus. Neuroendocrinology. 1985; 40(6):463-70. DOI: 10.1159/000124116. View

FOLLENIUS E . [Ultrastructure of the hypophysial aminergic nerves in the teleost Gasterosteus aculeatus degenerating after application of 6 hydroxydopamine]. Z Zellforsch Mikrosk Anat. 1972; 128(1):69-82. View

Pelletier G . Identification of ending containing dopamine and vasopressin in the rat posterior pituitary by a combination of radioautography and immunocytochemistry at the ultrastructural level. J Histochem Cytochem. 1983; 31(4):562-4. DOI: 10.1177/31.4.6827086. View

Abraham M, KIESELSTEIN M, Hilge V, Lison S . Extravascular circulation in the pituitary of Mugil cephalus (Teleostei). Cell Tissue Res. 1982; 225(3):567-79. DOI: 10.1007/BF00214805. View

Bloom F . Ultrastructural identification of catecholamine-containing central synaptic terminals. J Histochem Cytochem. 1973; 21(4):333-48. DOI: 10.1177/21.4.333. View

Zambrano D . The ultrastructure, catecholamine, and prolactin contents of the rostral pars distalis of the fish Mugil platanus after reserpine or 6-hydroxydopamine administration. Cell Tissue Res. 1975; 162(4):551-63. DOI: 10.1007/BF00209354. View

Kaul S, Vollrath L . The goldfish pituitary. II. Innervation. Cell Tissue Res. 1974; 154(2):231-49. DOI: 10.1007/BF00223166. View

Kah O, Dubourg P, CHAMBOLLE P, Calas A . Ultrastructural identification of catecholaminergic fibers in the goldfish pituitary. A high-resolution radioautographic study after in vitro 3H-dopamine administration. Cell Tissue Res. 1984; 238(3):621-6. DOI: 10.1007/BF00219880. View

10.

Nguyen-Legros J, Berger B, Alvarez C . High resolution radioautography of central dopaminergic fibers labelled in vitro with [3H]dopamine or [3H]norepinephrine. Brain Res. 1981; 213(2):265-76. DOI: 10.1016/0006-8993(81)90233-x. View

11.

Olivereau M, Aimar C, Olivereau J . PAS-positive cells of the pars intermedia are calcium-sensitive in the goldfish maintained in a hyposmotic milieu. Cell Tissue Res. 1980; 212(1):29-38. DOI: 10.1007/BF00234030. View

12.

Dubourg P, BURZAWA-GERARD E, CHAMBOLLE P, Kah O . Light and electron microscopic identification of gonadotrophic cells in the pituitary gland of the goldfish by means of immunocytochemistry. Gen Comp Endocrinol. 1985; 59(3):472-81. DOI: 10.1016/0016-6480(85)90407-1. View

13.

BALL J . Hypothalamic control of the pars distalis in fishes, amphibians, and reptiles. Gen Comp Endocrinol. 1981; 44(2):135-70. DOI: 10.1016/0016-6480(81)90243-4. View

14.

Hattori T, Fibiger H, McGeer P, Maler L . Analysis of the fine structure of the dopaminergic nigrostriatal projection by electron microscopic autoradiography. Exp Neurol. 1973; 41(3):599-611. DOI: 10.1016/0014-4886(73)90053-8. View

15.

Cuello A, Iversen L . Localization of tritiated dopamine in the median eminence of the rat hypothalamus by electron microscope autoradiography. Brain Res. 1973; 63:474-8. DOI: 10.1016/0006-8993(73)90127-3. View

16.

Chetverukhin V, Belenky M, POLENOV A . Quantitative radioautographic light and electron microscopic analysis of the localization of monoamines in the median eminence of the rat. I. Catecholamines. Cell Tissue Res. 1979; 203(3):469-85. DOI: 10.1007/BF00233275. View

17.

Kah O, CHAMBOLLE P, Thibault J, Geffard M . Existence of dopaminergic neurons in the preoptic region of the goldfish. Neurosci Lett. 1984; 48(3):293-8. DOI: 10.1016/0304-3940(84)90053-3. View

18.

Pickel V, Joh T, Reis D . Monoamine-synthesizing enzymes in central dopaminergic, noradrenergic and serotonergic neurons. Immunocytochemical localization by light and electron microscopy. J Histochem Cytochem. 1976; 24(7):792-306. DOI: 10.1177/24.7.8567. View

19.

Calas A . Morphological correlates of chemically specified neuronal interactions in the hypothalamo-hypophyseal area. Neurochem Int. 2010; 7(6):927-40. DOI: 10.1016/0197-0186(85)90141-x. View

20.

Cook H, Cook A, Peter R, Wilson S . Ultrastructural immunocytochemistry of growth hormone cells in the goldfish pituitary gland. Gen Comp Endocrinol. 1983; 50(3):348-53. DOI: 10.1016/0016-6480(83)90254-x. View