Low Molecular Weight Microtubule-associated Proteins Are Light Chains of Microtubule-associated Protein 1 (MAP 1)

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Mar 1

PMID 6572393

Citations 39

Authors

R B Vallee

S E Davis

Affiliations

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Abstract

Microtubule-associated protein 1 (MAP 1; Mr = 350,000) was analyzed by column chromatography of microtubule protein obtained from calf brain gray and white matter. Two low molecular weight proteins (LMW MAPs; Mr 28,000 and 30,000) were found to cochromatograph with MAP 1 under all conditions examined. MAP 1 and the LMW MAPs were purified from calf brain white matter as a complex containing approximately equimolar amounts of the three species. Urea (6 M) was used to remove the LMW MAPs from MAP 1. Binding of MAP 1 to microtubules was unaffected by urea and occurred with or without the LMW species. Electron microscopy of microtubules composed of purified tubulin and either MAP 1 preparation revealed that, like MAP 2, MAP 1 has the appearance of a filamentous arm on the microtubule surface.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

Gaskin F, Cantor C, Shelanski M . Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules. J Mol Biol. 1974; 89(4):737-55. DOI: 10.1016/0022-2836(74)90048-5. View

Sloboda R, Rudolph S, Rosenbaum J, Greengard P . Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein. Proc Natl Acad Sci U S A. 1975; 72(1):177-81. PMC: 432265. DOI: 10.1073/pnas.72.1.177. View

Borisy G, Marcum J, Olmsted J, Murphy D, Johnson K . Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro. Ann N Y Acad Sci. 1975; 253:107-32. DOI: 10.1111/j.1749-6632.1975.tb19196.x. View

Smith D, Jarlfors U, CAMERON B . Morphological evidence for the participation of microtubules in axonal transport. Ann N Y Acad Sci. 1975; 253:472-506. DOI: 10.1111/j.1749-6632.1975.tb19223.x. View

Weingarten M, Lockwood A, Hwo S, Kirschner M . A protein factor essential for microtubule assembly. Proc Natl Acad Sci U S A. 1975; 72(5):1858-62. PMC: 432646. DOI: 10.1073/pnas.72.5.1858. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Murphy D, Vallee R, Borisy G . Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules. Biochemistry. 1977; 16(12):2598-605. DOI: 10.1021/bi00631a004. View

10.

Fellous A, FRANCON J, Lennon A, Nunez J . Microtubule assembly in vitro. Purification of assembly-promoting factors. Eur J Biochem. 1977; 78(1):167-74. DOI: 10.1111/j.1432-1033.1977.tb11726.x. View

11.

Berkowitz S, Katagiri J, Binder H, Williams Jr R . Separation and characterization of microtubule proteins from calf brain. Biochemistry. 1977; 16(25):5610-7. DOI: 10.1021/bi00644a035. View

12.

Cleveland D, Hwo S, Kirschner M . Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin. J Mol Biol. 1977; 116(2):207-25. DOI: 10.1016/0022-2836(77)90213-3. View

13.

Vallee R, Borisy G . The non-tubulin component of microtubule protein oligomers. Effect on self-association and hydrodynamic properties. J Biol Chem. 1978; 253(8):2834-45. View

14.

Herzog W, Weber K . Fractionation of brain microtubule-associated proteins. Isolation of two different proteins which stimulate tubulin polymerization in vitro. Eur J Biochem. 1978; 92(1):1-8. DOI: 10.1111/j.1432-1033.1978.tb12716.x. View

15.

Pearse B . On the structural and functional components of coated vesicles. J Mol Biol. 1978; 126(4):803-12. DOI: 10.1016/0022-2836(78)90021-9. View

16.

Kim H, Binder L, Rosenbaum J . The periodic association of MAP2 with brain microtubules in vitro. J Cell Biol. 1979; 80(2):266-76. PMC: 2110341. DOI: 10.1083/jcb.80.2.266. View

17.

Zingsheim H, Herzog W, Weber K . Differences in surface morphology of microtubules reconstituted from pure brain tubulin using two different microtubule-associated proteins: the high molecular weight MAP 2 proteins and tau proteins. Eur J Cell Biol. 1979; 19(2):175-83. View

18.

Bell C, Fronk E, Gibbons I . Polypeptide subunits of dynein 1 from sea urchin sperm flagella. J Supramol Struct. 1979; 11(3):311-7. DOI: 10.1002/jss.400110305. View

19.

Vallee R . Structure and phosphorylation of microtubule-associated protein 2 (MAP 2). Proc Natl Acad Sci U S A. 1980; 77(6):3206-10. PMC: 349583. DOI: 10.1073/pnas.77.6.3206. View

20.

Ellisman M, PORTER K . Microtrabecular structure of the axoplasmic matrix: visualization of cross-linking structures and their distribution. J Cell Biol. 1980; 87(2 Pt 1):464-79. PMC: 2110738. DOI: 10.1083/jcb.87.2.464. View