A Microtubule-associated Protein (MAP1) Which is Expressed at Elevated Levels During Development of the Rat Cerebellum

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 May 1

PMID 4006912

Citations 21

Authors

R Calvert

B H Anderton

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibody (MAb) G10 labels a single high mol. wt. (HMW) band on Western blots of microtubule preparations from 2 day old rat brain. The G10 antigen is thermolabile and co-migrates with microtubule-associated protein (MAP)1 from young rat brain on low percentage (5%) polyacrylamide-SDS gels. The G10 antigen decreases by about five times from birth to adulthood in the rat cerebellum. The same single band is labelled on Western blots of homogenates of whole neonatal rat brain but no labelling is found using neonatal or adult kidney, lung or liver. We have therefore identified a brain-specific MAP1, designated MAP1(x). Immunofluorescence microscopy using MAb G10 on parasagittal sections of rat cerebella shows labelling of the newly formed molecular layer in 6 day old rats. Only a narrow band close to the pial surface is labelled in 18 day old animals, which disappears in the adult. Labelling of the cerebellar white matter found in young rats also disappears. Neurones but not flat cells in cerebellar cultures label with MAb G10. All staining patterns are consistent with an axonal distribution of the antigen. MAP1(x) may be part of a developmentally regulated microtubule structure.

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References

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Bennett G, DiLullo C . Expression of a neurofilament protein by the precursors of a subpopulation of ventral spinal cord neurons. Dev Biol. 1985; 107(1):94-106. DOI: 10.1016/0012-1606(85)90379-3. View

Rothman T, Specht L, Gershon M, Joh T, Teitelman G, Pickel V . Catecholamine biosynthetic enzymes are expressed in replicating cells of the peripheral but not the central nervous system. Proc Natl Acad Sci U S A. 1980; 77(10):6221-5. PMC: 350247. DOI: 10.1073/pnas.77.10.6221. View

Wood J, Anderton B . Monoclonal antibodies to mammalian neurofilaments. Biosci Rep. 1981; 1(3):263-8. DOI: 10.1007/BF01114913. View

Kuznetsov S, Rodionov V, Gelfand V, Rosenblat V . Purification of high-Mr microtubule proteins MAP1 and MAP2. FEBS Lett. 1981; 135(2):237-40. DOI: 10.1016/0014-5793(81)80790-9. View

Kuznetsov S, Rodionov V, Gelfand V, Rosenblat V . Microtubule-associated protein MAP1 promotes microtubule assembly in vitro. FEBS Lett. 1981; 135(2):241-4. DOI: 10.1016/0014-5793(81)80791-0. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Yamada K, Spooner B, Wessells N . Axon growth: roles of microfilaments and microtubules. Proc Natl Acad Sci U S A. 1970; 66(4):1206-12. PMC: 335807. DOI: 10.1073/pnas.66.4.1206. View

Seeds N, GILMAN A, Amano T, Nirenberg M . Regulation of axon formation by clonal lines of a neural tumor. Proc Natl Acad Sci U S A. 1970; 66(1):160-7. PMC: 286102. DOI: 10.1073/pnas.66.1.160. View

10.

Altman J . Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer. J Comp Neurol. 1972; 145(3):353-97. DOI: 10.1002/cne.901450305. View

11.

Altman J . Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layer. J Comp Neurol. 1972; 145(4):399-463. DOI: 10.1002/cne.901450402. View

12.

Weingarten M, Lockwood A, Hwo S, Kirschner M . A protein factor essential for microtubule assembly. Proc Natl Acad Sci U S A. 1975; 72(5):1858-62. PMC: 432646. DOI: 10.1073/pnas.72.5.1858. View

13.

Murphy D, Borisy G . Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro. Proc Natl Acad Sci U S A. 1975; 72(7):2696-700. PMC: 432837. DOI: 10.1073/pnas.72.7.2696. View

14.

Peterson G . A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977; 83(2):346-56. DOI: 10.1016/0003-2697(77)90043-4. View

15.

Gozes I, LITTAUER U . Tubulin microheterogeneity increases with rat brain maturation. Nature. 1978; 276(5686):411-3. DOI: 10.1038/276411a0. View

16.

Currie D, Dutton G, Cohen J . Monolayer cultures of perikarya isolated from postnatal rat cerebellum. Experientia. 1979; 35(3):345-7. DOI: 10.1007/BF01964343. View

17.

Vallee R . A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J Cell Biol. 1982; 92(2):435-42. PMC: 2112074. DOI: 10.1083/jcb.92.2.435. View

18.

Shaw G, Weber K . Differential expression of neurofilament triplet proteins in brain development. Nature. 1982; 298(5871):277-9. PMC: 7095408. DOI: 10.1038/298277a0. View

19.

Kilmartin J, Wright B, Milstein C . Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982; 93(3):576-82. PMC: 2112140. DOI: 10.1083/jcb.93.3.576. View

20.

Sherline P, Mascardo R . Epidermal growth factor-induced centrosomal separation: mechanism and relationship to mitogenesis. J Cell Biol. 1982; 95(1):316-22. PMC: 2112368. DOI: 10.1083/jcb.95.1.316. View