The Complexity of HLA-DS Molecules. A Homozygous Cell Line Expresses Multiple HLA-DS Alpha Chains

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1984 May 1

PMID 6425448

Citations 8

Authors

R W Karr

C Alber

S M Goyert

J Silver

R J Duquesnoy

Affiliations

Soon will be listed here.

Abstract

Ia molecules expressed by an HLA-DRw6 homozygous cell line were immunoprecipitated with anti-Ia allosera and monoclonal antibodies and analyzed by 2-D gel electrophoresis. The DRw6 homozygous cell line was shown to express two DS beta chains; this observation extends our previous finding that a DR5 homozygous cell line expresses two DS beta chains and suggests that the expression of at least two DS beta chains by DR homozygous cell lines is a generalized phenomenon. The data presented here document for the first time that a DR homozygous cell line expresses at least two DS alpha chains. Therefore, this cell line expresses at least two DS molecules with the potential for the expression of four DS molecules. In agreement with previous reports, the cell line was shown to express two DR beta chains and one DR alpha chain that combine to form two DR molecules. The molecular specificities of two MB1 allosera and two MB1 -like monoclonal antibodies were also compared in these studies. Both MB1 allosera isolated a single DS molecule, while the MB1 -like monoclonal antibodies isolated at least two DS molecules. Therefore, these studies document for the first time that anti-Ia reagents which are specific for the MB1 or MB1 -like determinants in population studies do not recognize the same Ia molecules in immunochemical studies. The data presented here for the expression of at least two DS alpha chains and the location of the MB1 allodeterminant on only one of multiple DS molecules are in agreement with recent studies at the gene level.

Citing Articles

Separation of four class II molecules from DR2 and DRw6 homozygous B lymphoid cell lines.

Maeda H, Hirata R Immunogenetics. 1984; 20(6):639-47.

PMID: 6392082 DOI: 10.1007/BF00430322.

Identification of two cis-encoded HLA-DQ molecules that carry distinct alloantigenic specificities.

Johnson J, Wank R J Exp Med. 1984; 160(5):1350-9.

PMID: 6208309 PMC: 2187512. DOI: 10.1084/jem.160.5.1350.

Nucleotide sequence of an HLA-DQ alpha chain derived from a DRw9 cell line: genetic and evolutionary implications.

Moriuchi J, Moriuchi T, Silver J Proc Natl Acad Sci U S A. 1985; 82(10):3420-4.

PMID: 3879967 PMC: 397787. DOI: 10.1073/pnas.82.10.3420.

Molecular diversity of HLA-DQ. DQ alpha and beta chain isoelectric point differences and their relation to serologically defined HLA-DQ allospecificities.

Bontrop R, Baas E, Otting N, Schreuder G, Giphart M Immunogenetics. 1987; 25(5):305-12.

PMID: 3570381 DOI: 10.1007/BF00404423.

A human cytotoxic lymphocyte subdividing the DQ1 antigen.

Fraser J, Strominger J Immunogenetics. 1987; 25(5):347-50.

PMID: 3552973 DOI: 10.1007/BF00404429.

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