Density Gradient Analysis of DNA Replicated During Bacillus Subtilis Sporulation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Oct 1

PMID 6413497

Citations 1

Authors

C Binnie

J G Coote

Affiliations

Soon will be listed here.

Abstract

Density gradient centrifugation was used to monitor DNA replication during sporulation of a 5-bromo-2'-deoxyuridine-tolerant, thymidine-requiring strain of Bacillus subtilis. DNA of heavy, intermediate, and light density was found in cells induced to sporulate in the presence of bromodeoxyuridine, but only intermediate DNA was detected in mature spores. Cells grown with bromodeoxyuridine until DNA was in the heavy form formed spores containing intermediate and light DNA when sporulated with thymidine alone.

Citing Articles

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PMID: 2118869 PMC: 1204096. DOI: 10.1093/genetics/125.4.703.

References

Sueoka N, Yoshikawa H . The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics. 1965; 52(4):747-57. PMC: 1210937. DOI: 10.1093/genetics/52.4.747. View

Laird C, Bodmer W . 5-bromouracil utilization by Bacillus subtilis. J Bacteriol. 1967; 94(4):1277-8. PMC: 276815. DOI: 10.1128/jb.94.4.1277-1278.1967. View

STERLINI J, MANDELSTAM J . Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969; 113(1):29-37. PMC: 1184601. DOI: 10.1042/bj1130029. View

RYTER A, Aubert J . [Autoradiographic study of DNA synthesis during sporulation of Bacillus subtilis]. Ann Inst Pasteur (Paris). 1969; 117(5):601-11. View

Dawes I, MANDELSTAM J . Sporulation of Bacillus subtilis in continuous culture. J Bacteriol. 1970; 103(3):529-35. PMC: 248122. DOI: 10.1128/jb.103.3.529-535.1970. View

EPHRATI-ELIZUR E, Borenstein S . Velocity of chromosome replication in thymine-requiring and independent strains of Bacillus subtilis. J Bacteriol. 1971; 106(1):58-64. PMC: 248644. DOI: 10.1128/jb.106.1.58-64.1971. View

Dawes I, Kay D, MANDELSTAM J . Determining effect of growth medium on the shape and position of daughter chromosomes and on sporulation in Bacillus subtilis. Nature. 1971; 230(5296):567-9. DOI: 10.1038/230567a0. View

MANDELSTAM J, STERLINI J, Kay D . Sporulation in Bacillus subtilis. Effect of medium on the form of chromosome replication and on initiation to sporulation in Bacillus subtilis. Biochem J. 1971; 125(2):635-41. PMC: 1178101. DOI: 10.1042/bj1250635. View

Holtzer H, Weintraub H, Mayne R, Mochan B . The cell cycle, cell lineages, and cell differentiation. Curr Top Dev Biol. 1972; 7:229-56. DOI: 10.1016/s0070-2153(08)60073-3. View

10.

MANDELSTAM J, Higgs S . Induction of sporulation during synchronized chromosome replication in Bacillus subtilis. J Bacteriol. 1974; 120(1):38-42. PMC: 245727. DOI: 10.1128/jb.120.1.38-42.1974. View

11.

Coote J . Comparative studies on induction of sporulation and synthesis of inducible enzymes in Bacillus subtilis. J Bacteriol. 1974; 120(3):1102-8. PMC: 245888. DOI: 10.1128/jb.120.3.1102-1108.1974. View

12.

Dancer B, MANDELSTAM J . Criteria for categorizing early biochemical events occurring during sporulation of Bacillus subtilis. J Bacteriol. 1975; 121(2):411-5. PMC: 245945. DOI: 10.1128/jb.121.2.411-415.1975. View

13.

Gillin F, Ganesan A . Control of chromosome replication in thymine-requiring strains of Bacillus subtilis 168. J Bacteriol. 1975; 123(3):1055-67. PMC: 235830. DOI: 10.1128/jb.123.3.1055-1067.1975. View

14.

Keynan A, Berns A, Dunn G, Young M, MANDELSTAM J . Resporulation of outgrowing Bacillus subtilis spores. J Bacteriol. 1976; 128(1):8-14. PMC: 232819. DOI: 10.1128/jb.128.1.8-14.1976. View

15.

Coote J . Interference by bromodeoxyuridine with differentiation in a prokaryote. Nature. 1977; 267(5612):635-7. DOI: 10.1038/267635a0. View

16.

Pragnell I, Arndt-Jovin D, Jovin T, FAGG B, Ostertag W . Commitment to differentiation in Friend cells and initiation of globin mRNA synthesis occurs during the G1 phase of the cell cycle. Exp Cell Res. 1980; 125(2):459-70. DOI: 10.1016/0014-4827(80)90140-8. View

17.

Sargent M . A procedure for isolating high quality DNA from spores of Bacillus subtilis 168. J Gen Microbiol. 1980; 116(2):511-4. DOI: 10.1099/00221287-116-2-511. View

18.

Sargent M . Chromosome replication in sporulating cells of Bacillus subtilis. J Bacteriol. 1980; 142(2):491-8. PMC: 294010. DOI: 10.1128/jb.142.2.491-498.1980. View

19.

SCHILDKRAUT C, MARMUR J, Doty P . Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962; 4:430-43. DOI: 10.1016/s0022-2836(62)80100-4. View

20.

Wake R, Baldwin R . Physical studies on the replication of DNA in vitro. J Mol Biol. 1962; 5:201-16. DOI: 10.1016/s0022-2836(62)80084-9. View