Resporulation of Outgrowing Bacillus Subtilis Spores

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 Oct 1

PMID 824279

Citations 8

Authors

A Keynan

A A Berns

G Dunn

M Young

J MANDELSTAM

Affiliations

Soon will be listed here.

Abstract

Germinated spores of Bacillus subtilis were incubated in outgrowth medium and tested periodically for capacity to sporulate when suspended in sporulation medium. Concurrent measurements were made of deoxyribonucleic acid (DNA) content and numbers of cell division septa and nucleoids. Sporulation potential is shown to reach a peak at about 110 min at which time the chromosomes are probably well into the second round of replication. Experiments with nalidixic acid show that sporulation potential can be generated in the outgrowth medium even when DNA synthesis is largely prevented. Further experiments show that nalidixic acid apparently does not prevent the formation of DNA initiation complexes, which can subsequently function after resuspension in the sporulation medium. The results support those previously obtained with a temperature-sensitive DNA mutant which indicated that sporulation could only be induced at a specific stage of chromosome replication, and then only if the cells are in a state of nutritional "step-down".

Citing Articles

Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis.

Errington J Microbiol Rev. 1993; 57(1):1-33.

PMID: 8464402 PMC: 372899. DOI: 10.1128/mr.57.1.1-33.1993.

Chromosome replication in sporulating cells of Bacillus subtilis.

Sargent M J Bacteriol. 1980; 142(2):491-8.

PMID: 6769901 PMC: 294010. DOI: 10.1128/jb.142.2.491-498.1980.

Cell division of cycle of Bacillus subtilis: evidence of variability in period D.

Holmes M, Rickert M, PIERUCCI O J Bacteriol. 1980; 142(1):254-61.

PMID: 6768710 PMC: 293941. DOI: 10.1128/jb.142.1.254-261.1980.

Density gradient analysis of DNA replicated during Bacillus subtilis sporulation.

Binnie C, Coote J J Bacteriol. 1983; 156(1):466-70.

PMID: 6413497 PMC: 215112. DOI: 10.1128/jb.156.1.466-470.1983.

Bacterial spores and chemical sporicidal agents.

Russell A Clin Microbiol Rev. 1990; 3(2):99-119.

PMID: 2187595 PMC: 358146. DOI: 10.1128/CMR.3.2.99.

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