Protective Immunization of the Squirrel Monkey Against Asexual Blood Stages of Plasmodium Falciparum by Use of Parasite Protein Fractions

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Jan 1

PMID 6364136

Citations 17

Authors

P Dubois

J P Dedet

T Fandeur

C Roussilhon

M Jendoubi

S Pauillac

O Mercereau-Puijalon

L Pereira da Silva

Affiliations

Soon will be listed here.

Abstract

We had previously shown that two polypeptides of Plasmodium falciparum are preferentially recognized by antibodies of resistant squirrel monkeys Saimiri sciureus. Free parasites were isolated from synchronized cultures on human erythrocytes initially inoculated with infected Saimiri erythrocytes. Crude extracts were prepared from mature schizont stages and electrophoresed on preparative NaDodSO4/polyacrylamide gels. Two groups of five monkeys were immunized with protein fractions eluted from the 75- and 100-kilodalton regions of the gels. Strong protection against challenge by the homologous strain of P. falciparum was induced in both groups. Analysis of specific anti-malarial antibodies revealed a homogeneous response of all the animals against a few polypeptides of the mature parasite.

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