A Mitochondrial Ribosomal RNA Mutation and Its Nuclear Suppressors

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1984 Jan 1

PMID 6363879

Citations 6

Authors

V Contamine

M Bolotin-Fukuhara

Affiliations

Soon will be listed here.

Abstract

We have isolated cold-resistant revertants from a mitochondrial cold-sensitive mutation, cs909, localized in the 21S ribosomal RNA gene. Two types of revertants have been isolated: (1) strong revertants which were shown to be due to a single, nuclear, dominant suppressor; (2) weak revertants which are all due to the presence of a single, nuclear, recessive suppressor. The recessive suppressor, when separated from the mitochondrial mutation, itself confers a cold-sensitive phenotype, that is, there is a mutual suppression between the mitochondrial cold-sensitive mutation and the nuclear cold-sensitive mutation. The suppressor by itself produces modified ribosomes and therefore probably codes for an element of the mitochondrial ribosome such as a ribosomal protein.

Citing Articles

MBR1 and MBR3, two related yeast genes that can suppress the growth defect of hap2, hap3 and hap4 mutants.

Nguyen C, Reisdorf P, Lemeignan B, Bolotin-Fukuhara M Mol Gen Genet. 1994; 243(5):575-83.

PMID: 8208248 DOI: 10.1007/BF00284206.

Suppression of a mitochondrial point mutation in a tRNA gene can cast light on the mechanisms of 3' end-processing.

Rinaldi T, Francisci S, Zennaro E, Frontali L, Bolotin-Fukuhara M Curr Genet. 1994; 25(5):451-5.

PMID: 7521797 DOI: 10.1007/BF00351785.

Nuclear mutations affecting the stability of the mitochondrial genome in S. cerevisiae.

Rayko E, Goursot R Curr Genet. 1985; 10(3):171-7.

PMID: 3916806 DOI: 10.1007/BF00798746.

Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.

Bolotin-Fukuhara M Nucleic Acids Res. 1988; 16(19):9299-306.

PMID: 3050898 PMC: 338707. DOI: 10.1093/nar/16.19.9299.

Nuclear and mitochondrial revertants of a mitochondrial mutant with a defect in the ATP synthetase complex.

Hefta L, Lewin A, Bolotin-Fukuhara M Mol Gen Genet. 1987; 207(1):106-13.

PMID: 2885722 DOI: 10.1007/BF00331497.

References

Sor F, Fukuhara H . Complete DNA sequence coding for the large ribosomal RNA of yeast mitochondria. Nucleic Acids Res. 1983; 11(2):339-48. PMC: 325718. DOI: 10.1093/nar/11.2.339. View

Sor F, Faye G . Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. II. Biochemistry. Mol Gen Genet. 1979; 177(1):47-56. DOI: 10.1007/BF00267252. View

Tzagoloff A, Akai A, Needleman R . Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions. J Bacteriol. 1975; 122(3):826-31. PMC: 246131. DOI: 10.1128/jb.122.3.826-831.1975. View

Faye G, Kujawa C, Dujon B, Bolotin-Fukuhara M, Wolf K, Fukuhara H . Localization of the gene coding for the mitochondrial 16 S ribosomal RNA using rho- mutants of Saccharomyces cerevisiae. J Mol Biol. 1975; 99(1):203-17. DOI: 10.1016/s0022-2836(75)80168-9. View

Faye G, Kujawa C, Fukuhara H . Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae. J Mol Biol. 1974; 88(1):185-203. DOI: 10.1016/0022-2836(74)90304-0. View

Brimacombe R, Maly P, Zwieb C . The structure of ribosomal RNA and its organization relative to ribosomal protein. Prog Nucleic Acid Res Mol Biol. 1983; 28:1-48. DOI: 10.1016/s0079-6603(08)60081-1. View

Devenish R, Englisn K, Hall R, Linnase A, Lukins H . Biogenesis of mitochondria 49 identification and mapping of a new mitochondrial locus (tsr1) which maps within polar region of yeast mitochondrial genome. Mol Gen Genet. 1978; 161(3):251-9. DOI: 10.1007/BF00330998. View

Terpstra P, Zanders E, Butow R . The association of var1 with the 38 S mitochondrial ribosomal subunit in yeast. J Biol Chem. 1979; 254(24):12653-61. View

Groot G, Mason T, Van Harten-Loosbroek N . Var1 is associated with the small ribosomal subunit of mitochondrial ribosomes in yeast. Mol Gen Genet. 1979; 174(3):339-42. DOI: 10.1007/BF00267808. View

10.

Julou C, Bolotin-Fukuhara M . Genetics of mitochondrial ribosomes of yeast: mitochondrial lethality of a double mutant carrying two mutations of the 21S ribosomal RNA gene. Mol Gen Genet. 1982; 188(2):256-60. DOI: 10.1007/BF00332684. View

11.

Masselot M, DE ROBICHON-SZULMAJSTER H . Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet. 1975; 139(2):121-32. DOI: 10.1007/BF00264692. View

12.

Bolotin-Fukuhara M . Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. I. Genetics. Mol Gen Genet. 1979; 177(1):39-46. DOI: 10.1007/BF00267251. View

13.

Coen D, Deutsch J, Netter P, Petrochilo E, SLONIMSKI P . Mitochondrial genetics. I. Methodology and phenomenology. Symp Soc Exp Biol. 1970; 24:449-96. View

14.

Bolotin-Fukuhara M, Fay G, Fukuhara H . Temperature-sensitive respiratory-deficient mitochondrial mutations: isolation and genetic mapping. Mol Gen Genet. 1977; 152(3):295-305. DOI: 10.1007/BF00693083. View

15.

Faye G, Sor F . Analysis of mitochondrial ribosomal proteins of Saccharomyces cerevisiae by two dimensional polyacrylamide gel electrophoresis. Mol Gen Genet. 1977; 155(1):27-34. DOI: 10.1007/BF00268557. View

16.

Singh A, Mason T, Zimmermann R . A cold-sensitive cytoplasmic mutation of Saccharomyces cerevisiae affecting assembly of the mitochondrial 50S ribosomal subunit. Mol Gen Genet. 1978; 161(2):143-51. DOI: 10.1007/BF00274184. View