Assembly of the Mitochondrial Membrane System: Isolation of Nuclear and Cytoplasmic Mutants of Saccharomyces Cerevisiae with Specific Defects in Mitochondrial Functions

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1975 Jun 1

PMID 168180

Citations 48

Authors

A Tzagoloff

A Akai

R B Needleman

Affiliations

Soon will be listed here.

Abstract

A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c reductase, cytochrome oxidase, and adenosine triphosphatase. In addition, two cytoplasmic mutants have been found whose primary defect is in cytochrome oxidase, and others have been found that show variable degrees of abnormalities in their mitochondrial translation products.

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References

Sherman F, SLONIMSKI P . RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964; 90:1-15. DOI: 10.1016/0304-4165(64)90113-8. View

EPHRUSSI B, SLONIMSKI P, PERRODIN G . [The adaptative synthesis of cytochromes in baker's yeast]. Biochim Biophys Acta. 1950; 6(2):256-67. DOI: 10.1016/0006-3002(50)90098-9. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Sherman F, Stewart J, Parker J, Inhaber E, Shipman N, Putterman G . The mutational alteration of the primary structure of yeast iso-1-cytochrome c. J Biol Chem. 1968; 243(20):5446-56. View

Subik J, KUZELA S, Kolarov J, Kovac L, Lachowicz T . Oxidative phosphorylation in yeast. VI. ATPase activity and protein synthesis in mitochondria isolated from nuclear mutants deficient in cytochromes. Biochim Biophys Acta. 1970; 205(3):513-9. DOI: 10.1016/0005-2728(70)90117-9. View

Tzagoloff A, Meagher P . Assesmbly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem. 1972; 247(2):594-603. View

Weiss H, Ziganke B . Cytochrome b in Neurospora crassa mitochondria. Site of translation of the heme protein. Eur J Biochem. 1974; 41(1):63-71. DOI: 10.1111/j.1432-1033.1974.tb03244.x. View

Weiss H, Sebald W, Bucher T . Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa. Eur J Biochem. 1971; 22(1):19-26. DOI: 10.1111/j.1432-1033.1971.tb01509.x. View

Subik J, Kovac L, Kolarov J . Oxidative phosphorylation in yeast. X. Phosphorylation ability of mutants deficient in cytochromes a and b. Biochim Biophys Acta. 1972; 283(1):146-54. DOI: 10.1016/0005-2728(72)90106-5. View

10.

Mason T, Schatz G . Cytochrome c oxidase from bakers' yeast. II. Site of translation of the protein components. J Biol Chem. 1973; 248(4):1355-60. View

11.

Rubin M, Tzagoloff A . Assembly of the mitochondrial membrane system. X. Mitochondrial synthesis of three of the subunit proteins of yeast cytochrome oxidase. J Biol Chem. 1973; 248(12):4275-9. View

12.

EBNER E, MENNUCCI L, Schatz G . Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. I. Effect of nuclear mutations on mitochondrial protein synthesis. J Biol Chem. 1973; 248(15):5360-8. View

13.

Flury U, Mahler H, Feldman F . A novel respiration-deficient mutant of Saccharomyces cerevisiae. I. Preliminary characterization of phenotype and mitochondrial inheritance. J Biol Chem. 1974; 249(19):6130-7. View

14.

Putrament A, Baranowska H, Prazmo W . Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet. 1973; 126(4):357-66. DOI: 10.1007/BF00269445. View

15.

Sherman F, Stewart J, MARGOLIASH E, Parker J, Campbell W . The structural gene for yeast cytochrome C. Proc Natl Acad Sci U S A. 1966; 55(6):1498-504. PMC: 224350. DOI: 10.1073/pnas.55.6.1498. View

16.

Schatz G, Saltzgaber J . Protein synthesis by yeast promitochondria in vivo. Biochem Biophys Res Commun. 1969; 37(6):996-1001. DOI: 10.1016/0006-291x(69)90230-7. View

17.

Goldring E, Grossman L, Krupnick D, Cryer D, MARMUR J . The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J Mol Biol. 1970; 52(2):323-35. DOI: 10.1016/0022-2836(70)90033-1. View

18.

KUZELA S, Grecna E . Lack of amino acid incorporation by isolated mitochondria from respiratory-deficient cytoplasmic yeast mutants. Experientia. 1969; 25(7):776-7. DOI: 10.1007/BF01897625. View