Yeast Ribosomal Proteins: VII. Cytoplasmic Ribosomal Proteins from Schizosaccharomyces Pombe

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1983 Jan 1

PMID 6355773

Citations 16

Authors

E Otaka

K Higo

T Itoh

Affiliations

Soon will be listed here.

Abstract

The cytoplasmic ribosomal proteins from a fission yeast Schizosaccharomyces pombe were analysed by two-dimensional polyacrylamide gel electrophoresis. Seventy-three protein species were identified in the 80S ribosome, and named SP-S1 to SP-S33 and SP-L1 to SP-L40 in the small and large subunits, respectively. Many of these proteins could be correlated to those of Saccharomyces cerevisiae on the basis of their electrophoretic mobilities. Eleven proteins were isolated from the 80S ribosome, and their amino acid compositions were determined. Of these, SP-S6, SP-L1, SP-L12, SP-L15, SP-L17, SP-L27, SP-L36 and SP-L40c and d were sequenced from their amino-termini. SP-S28 and SP-L2 appear to have their amino-termini blocked. These results were compared with the data available for the S. cerevisiae and rat liver ribosomal proteins. The S. cerevisiae counterparts of the eight proteins mentioned above were found to be YS4, YL1, YL10, YL14, YL35, YL40 and YL44c and d, respectively. The rat liver counterparts of SP-S6, SP-L1, SP-L27 and SP-L40c and d were the rat S6, L4, L37 and P2, respectively. Comparison of the partial sequences of these ribosomal proteins suggests that these two yeasts are relatively far apart, phylogenetically.

Citing Articles

Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.

Otaka E, Higo K, Itoh T Mol Gen Genet. 1984; 195(3):544-6.

PMID: 18782943 DOI: 10.1007/BF00341461.

Structure/function analysis of yeast ribosomal protein L2.

Meskauskas A, Russ J, Dinman J Nucleic Acids Res. 2008; 36(6):1826-35.

PMID: 18263608 PMC: 2330241. DOI: 10.1093/nar/gkn034.

Phylogenesis of fission yeasts. Contradictions surrounding the origin of a century old genus.

Sipiczki M Antonie Van Leeuwenhoek. 1995; 68(2):119-49.

PMID: 8546451 DOI: 10.1007/BF00873099.

The genes for yeast ribosomal proteins S24 and L46 are adjacent and divergently transcribed.

Leer R, Kraakman P, Mager W, Planta R Nucleic Acids Res. 1985; 13(3):701-9.

PMID: 4000930 PMC: 341029. DOI: 10.1093/nar/13.3.701.

Sequence of the cDNA for one acidic ribosomal protein of Schizosaccharomyces pombe.

Beltrame M, Bianchi M Nucleic Acids Res. 1987; 15(21):9089.

PMID: 3684587 PMC: 306435. DOI: 10.1093/nar/15.21.9089.

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