Yeast Ribosomal Proteins. I. Characterization of Cytoplasmic Ribosomal Proteins by Two-dimensional Gel Electrophoresis

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Jul 4

PMID 355832

Citations 17

Authors

E Otaka

K Kobata

Affiliations

Soon will be listed here.

Abstract

The cytoplasmic 80s ribosomal proteins from the cells of yeast Sachharomyces cerevisiae were analysed by SDS two-dimensional polyacrylamide gel electrophoresis. Seventyfour proteins were identified and consecutively numbered from 1 to 74. Upon oxidation of the 80s proteins with performic acid, ten proteins (no. 15, 20, 35, 40, 44, 46, 49, 51, 54 and 55) were dislocated on the gel without change of the total number of protein spots. Five proteins (no. 8, 14, 16, 36 and 74) were phosphorylated in vivo as seen in 32P-labelling experiments. The large and small subunits separated in low magnesium medium were analyzed by the above gel electrophoresis. At least forty-five and twenty-eight proteins were assumed to be in the large and small subunits, respectively. All proteins found in the 80s ribosomes, except for no. 3, were detected in either subunit without appearance of new spots. The acidic protein no. 3 seems to be lost during subunit dissociation.

Citing Articles

Effects of Gαi and Gαz protein knockdown on alpha-adrenergic and cannabinoid CB receptor regulation of MEK-ERK and FADD pathways in mouse cerebral cortex.

Ramos-Miguel A, Sanchez-Blazquez P, Garcia-Sevilla J Pharmacol Rep. 2021; 73(4):1122-1135.

PMID: 33641090 DOI: 10.1007/s43440-021-00240-4.

Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae.

Stocklein W, Piepersberg W Curr Genet. 2013; 1(3):177-83.

PMID: 24189656 DOI: 10.1007/BF00390941.

RPL41, a small ribosomal peptide deregulated in tumors, is essential for mitosis and centrosome integrity.

Wang S, Huang J, He J, Wang A, Xu S, Huang S Neoplasia. 2010; 12(3):284-93.

PMID: 20234822 PMC: 2838445. DOI: 10.1593/neo.91610.

Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans.

Blakely G, Hekman J, Chakraburtty K, Williamson P J Bacteriol. 2001; 183(7):2241-8.

PMID: 11244063 PMC: 95130. DOI: 10.1128/JB.183.7.2241-2248.2001.

Nuclear export of the small ribosomal subunit requires the ran-GTPase cycle and certain nucleoporins.

Moy T, Silver P Genes Dev. 1999; 13(16):2118-33.

PMID: 10465789 PMC: 316945. DOI: 10.1101/gad.13.16.2118.

References

Zinker S, Warner J . The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins. J Biol Chem. 1976; 251(6):1799-807. View

Hebert J, Pierre M, Loeb J . Phosphorylation in vitro and in vivo of ribosomal proteins from Saccharomyces cerevisia. Eur J Biochem. 1977; 72(1):167-74. DOI: 10.1111/j.1432-1033.1977.tb11236.x. View

CRAVEN G, Voynow P, Hardy S, Kurland C . The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins. Biochemistry. 1969; 8(7):2906-15. DOI: 10.1021/bi00835a032. View

Sherton C, Wool I . Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis. J Biol Chem. 1972; 247(14):4460-7. View

Pratt H, Cox R . Proteins from biologically active ribosomal subparticles of Xenopus leavis. Biochim Biophys Acta. 1973; 310(1):188-204. DOI: 10.1016/0005-2795(73)90022-6. View

Tsurugi K, Collatz E, Wool E, Lin A . Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, L33, L35', L37, and L39. J Biol Chem. 1976; 251(24):7940-6. View

Ishiguro J . Study on proteins from yeast cytoplasmic ribosomes by two-dimensional gel electrophoresis. Mol Gen Genet. 1976; 145(1):73-9. DOI: 10.1007/BF00331560. View

Morrison C, Garrett R, Zeichhardt H, Stoffler G . Proteins occurring at, or near, the subunit interface of E. coli ribosomes. Mol Gen Genet. 1973; 127(4):359-68. DOI: 10.1007/BF00267106. View

Terao K, Ogata K . Studies on structural proteins of the rat liver ribosomes. I. Molecular wights of the proteins of large and small subunits. Biochim Biophys Acta. 1975; 402(2):214-29. DOI: 10.1016/0005-2787(75)90041-6. View

10.

Gorenstein C, Warner J . Coordinate regulation of the synthesis of eukaryotic ribosomal proteins. Proc Natl Acad Sci U S A. 1976; 73(5):1547-51. PMC: 430334. DOI: 10.1073/pnas.73.5.1547. View

11.

Welfle H, Stahl J, BIELKA H . Studies on proteins of animal ribosomes. 13. Enumeration of ribosomal proteins of rat liver. FEBS Lett. 1972; 26(1):228-32. DOI: 10.1016/0014-5793(72)80579-9. View

12.

Kanda F, Ochiai H, Iwabuchi M . Molecular-weight determinations and stoichiometric measurements of 40-S and 60-S ribosomal proteins of the cellular slime mold Dictyostelium discoideum. Eur J Biochem. 1974; 44(2):469-79. DOI: 10.1111/j.1432-1033.1974.tb03505.x. View

13.

Collatz E, Lin A, Stoffler G, Tsurugi K, Wool I . Group fractionation of eukaryotic ribosomal proteins. J Biol Chem. 1976; 251(6):1808-16. View

14.

Terao K, Ogata K . Characterization of the proteins of the small subunits of rat liver ribosomes. Biochim Biophys Acta. 1972; 285(2):473-82. DOI: 10.1016/0005-2795(72)90335-2. View

15.

Peeters B, Vanduffel L, Depuydt A, ROMBAUTS W . The number and size of the proteins in the subunits of human placental ribosomes. FEBS Lett. 1973; 36(2):217-21. DOI: 10.1016/0014-5793(73)80372-2. View

16.

Wittmann-Liebold B, Dzionara M . Comparison of amino acid sequences among ribosomal proteins of Escherichia coli. FEBS Lett. 1976; 61(1):14-9. DOI: 10.1016/0014-5793(76)80160-3. View

17.

Lin A, Wool I . The molecular weights of rat liver ribosomal proteins determined by "three-dimensional" polyacrylamide gel electrophoresis. Mol Gen Genet. 1974; 134(1):1-6. DOI: 10.1007/BF00332807. View

18.

Kruiswijk T, Planta R . Further analysis of the protein composition of yeast ribosomes. FEBS Lett. 1975; 58(1):102-5. DOI: 10.1016/0014-5793(75)80235-3. View

19.

Westermann P, BIELKA H . Studies on proteins of animal ribosomes. XV. Proteins of the small subunit of rat liver ribosomes: isolation, amino acid composition, tryptic peptides and molecular weights. Mol Gen Genet. 1973; 126(4):349-56. DOI: 10.1007/BF00269444. View

20.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View