Isolation and Characterization of Fourteen Ribosomal Proteins from Small Subunits of Yeast

Overview

Journal Biochemistry

Specialty Biochemistry

Date 1979 Sep 18

PMID 385049

Citations 8

Authors

K Higo

E Otaka

Affiliations

Soon will be listed here.

Abstract

A method for preparation of a large amount of ribosomal subunits from Saccharomyces cerevisiae by a Ti-15 zonal rotor is described. The proteins of the small subunits (ca. 50 000 A260 units) were separated into 22 fractions by chromatography on carboxymethylcellulose columns. Fourteen proteins were then purified from the ten chromatographic fractions by filtration through Sephadex G-100 or Sephacryl S--200. The isolated proteins are YP 6, YP 7, YP 9, YP 12, YP 14', YP 14'', YP 28, YP 38, YP 45, YP 50, YP 52, YP 58, YP 63, and YP 70. The molecular weight and amino acid compositions of these proteins are presented.

Citing Articles

Genetic and biochemical characterization of antisuppressor mutants in the yeast Saccharomyces cerevisiae.

Ishiguro J Curr Genet. 2013; 4(3):197-204.

PMID: 24185993 DOI: 10.1007/BF00420499.

Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.

Otaka E, Higo K, Itoh T Mol Gen Genet. 1984; 195(3):544-6.

PMID: 18782943 DOI: 10.1007/BF00341461.

Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.

Wu J, Beniac D, Harauz G Mol Cell Biochem. 1995; 143(1):21-34.

PMID: 7776955 DOI: 10.1007/BF00925923.

Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe.

Otaka E, Higo K, Itoh T Mol Gen Genet. 1983; 191(3):519-24.

PMID: 6355773 DOI: 10.1007/BF00425772.

Yeast ribosomal protein S33 is encoded by an unsplit gene.

Leer R, Schoppink P, Cornelissen M, Cohen L, Mager W, Planta R Nucleic Acids Res. 1983; 11(22):7759-68.

PMID: 6196722 PMC: 326528. DOI: 10.1093/nar/11.22.7759.