Identification and Mapping of Polypeptides Encoded by the P3HR-1 Strain of Epstein-Barr Virus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Jul 1

PMID 6330747

Citations 16

Authors

L K Cohen

S H Speck

B E Roberts

J L Strominger

Affiliations

Soon will be listed here.

Abstract

The Epstein-Barr virus (EBV)-specified polypeptides induced upon viral replication in the P3HR-1 cell line have been examined by immunoprecipitation with a high-titer human anti-EBV serum. Twenty-five predominant polypeptides were identified in cell extracts, whereas 18 polypeptides were precipitated from cell-free translation reactions directed by total mRNA. Hybrid selection of mRNA to the BamHI DNA clones of the EBV genome and immunoprecipitation of the corresponding cell-free translation products revealed 98 EBV-specified polypeptides and their coding location along the viral genome. In addition, the viral polypeptides that bind reversibly to DNA-cellulose have been characterized and the deduced map locations of this functional group of EBV-specified polypeptides is presented.

Citing Articles

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