DNA-binding Proteins Induced by Herpes Simplex Virus Type 2 in HEp-2 Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1976 Aug 1

PMID 183021

Citations 53

Authors

D J Purifoy

K L Powell

Affiliations

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Abstract

Affinity chromatography on single-stranded and double-stranded DNA-cellulose indicates that 12 proteins previously identified from herpes simplex virus type 2-infected cells, ranging in molecular weight from 28 X 10(3) to 186 X 10(3), bind to DNA-cellulose. The DNA-binding proteins found in infected cells differed in relative binding strengths for denatured DNA-cellulose. The virus specificity of these DNA-binding proteins was further studied by comparison with DNA-binding proteins isolated from mock-infected cells, and by immunoprecipitation of infected-cell DNA-binding proteins with antisera specific for viral antigens. The promise this technique holds for the purification and study of polypeptides involved in virus DNA replication, recombination, or repair is discussed.

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