Chromosomal Location and Cloning of the Gene (trmD) Responsible for the Synthesis of TRNA (m1G) Methyltransferase in Escherichia Coli K-12

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 6298573

Citations 39

Authors

A S Bystrom

G R Bjork

Affiliations

Soon will be listed here.

Abstract

The trmD gene, which governs the formation of 1-methyl-guanosine(m1G) in transfer ribonucleic acid (tRNA), has been located by phage P1 transduction at 56 min on the chromosomal map of Escherichia coli. Cotransduction to tyrA at 56 min is 80%. From the Clarke and Carbon collection a ColE1-tyrA+ hybrid plasmid was isolated, which carried the trmD+ gene and was shown to over-produce the tRNA(m1G)methyltransferase. By subcloning restriction enzyme fragments in vitro, the trmD+ gene was located to a 3.4 kb DNA fragment 6.5 kb clockwise from the tyrA+ gene. The mutation trmD1, which renders the tRNA(m1G)methyltransferase temperature-sensitive both in vivo and in vitro could be complemented by trmD+ plasmids. These results suggest that the gene trmD+ is the structural gene for the tRNA(m1G)methyltransferase (EC 2.1.1.3.1).

Citing Articles

Escherichia coli tRNA (Gm18) methyltransferase (TrmH) requires the correct localization of its methylation site (G18) in the D-loop for efficient methylation.

Kohno Y, Ito A, Okamoto A, Yamagami R, Hirata A, Hori H J Biochem. 2023; 175(1):43-56.

PMID: 37844264 PMC: 11640301. DOI: 10.1093/jb/mvad076.

Transfer RNA Modification Enzymes with a Thiouridine Synthetase, Methyltransferase and Pseudouridine Synthase (THUMP) Domain and the Nucleosides They Produce in tRNA.

Hori H Genes (Basel). 2023; 14(2).

PMID: 36833309 PMC: 9957541. DOI: 10.3390/genes14020382.

Real-Time Messenger RNA Dynamics in .

Sattler L, Graumann P Front Microbiol. 2021; 12:760857.

PMID: 34867890 PMC: 8637298. DOI: 10.3389/fmicb.2021.760857.

Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.

Zhang J Wiley Interdiscip Rev RNA. 2020; 11(6):e1600.

PMID: 32633085 PMC: 7583486. DOI: 10.1002/wrna.1600.

Distinct Modified Nucleosides in tRNA from the Hyperthermophilic Archaeon Thermococcus kodakarensis and Requirement of tRNA mG10/mG10 Methyltransferase (Archaeal Trm11) for Survival at High Temperatures.

Hirata A, Suzuki T, Nagano T, Fujii D, Okamoto M, Sora M J Bacteriol. 2019; 201(21).

PMID: 31405913 PMC: 6779453. DOI: 10.1128/JB.00448-19.

References

Csonka L, Clark A . Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains. J Bacteriol. 1980; 143(1):529-30. PMC: 294283. DOI: 10.1128/jb.143.1.529-530.1980. View

Blank H, Soll D . The nucleotide sequence of two leucine tRNA species from Escherichia coli K12. Biochem Biophys Res Commun. 1971; 43(5):1192-7. DOI: 10.1016/0006-291x(71)90589-4. View

Zurawski G, Brown K . Directed integration of bacteriophage lambda in an F lac transposition Hfr strain of Escherichia coli: isolation and characterization of specialized transducing phages for the phenylalanine and tyrosine operons. J Mol Biol. 1976; 102(2):311-24. DOI: 10.1016/s0022-2836(76)80056-3. View

Gauss D, Sprinzl M . Compilation of tRNA sequences. Nucleic Acids Res. 1981; 9(1):r1-23. PMC: 326680. View

Dube S, Marcker K, Yudelevich A . The nucleotide sequence of a leucine transfer RNA from E. coli. FEBS Lett. 1970; 9(3):168-170. DOI: 10.1016/0014-5793(70)80345-3. View

Bachmann B, Low K . Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980; 44(1):1-56. PMC: 373233. DOI: 10.1128/mr.44.1.1-56.1980. View

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

Ny T, Thomale J, Hjalmarsson K, NASS G, Bjork G . Non-coordinate regulation of enzymes involved in transfer RNA metabolism in Escherichia coli. Biochim Biophys Acta. 1980; 607(2):277-84. DOI: 10.1016/0005-2787(80)90080-5. View

Clarke L, Carbon J . A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell. 1976; 9(1):91-9. DOI: 10.1016/0092-8674(76)90055-6. View

10.

Clark A, MARGULIES A . ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965; 53:451-9. PMC: 219534. DOI: 10.1073/pnas.53.2.451. View

11.

Smolar N, Hellman U, Svensson I . Two transfer RNA (1-methylguanine) methylases from yeast. Nucleic Acids Res. 1975; 2(6):993-1004. PMC: 343485. DOI: 10.1093/nar/2.6.993. View

12.

Durwald H, HOFFMANN-BERLING H . Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968; 34(2):331-46. DOI: 10.1016/0022-2836(68)90257-x. View

13.

Bjork G . Transductional mapping of gene trmA responsible for the production of 5-methyluridine in transfer ribonucleic acid of Escherichia coli. J Bacteriol. 1975; 124(1):92-8. PMC: 235869. DOI: 10.1128/jb.124.1.92-98.1975. View

14.

Barrell B, Seidman J, Guthrie C, McClain W . Transfer RNA biosynthesis: the nucleotide sequence of a precursor to serine and proline transfer RNAs. Proc Natl Acad Sci U S A. 1974; 71(2):413-6. PMC: 388016. DOI: 10.1073/pnas.71.2.413. View

15.

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

16.

Jorgensen R, Rothstein S, Reznikoff W . A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979; 177(1):65-72. DOI: 10.1007/BF00267254. View

17.

Zurawski G, Brown K, Killingly D, Yanofsky C . Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli. Proc Natl Acad Sci U S A. 1978; 75(9):4271-5. PMC: 336095. DOI: 10.1073/pnas.75.9.4271. View

18.

Ny T, Bjork G . Cloning and restriction mapping of the trmA gene coding for transfer ribonucleic acid (5-methyluridine)-methyltransferase in Escherichia coli K-12. J Bacteriol. 1980; 142(2):371-9. PMC: 293980. DOI: 10.1128/jb.142.2.371-379.1980. View

19.

Sutcliffe J . Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A. 1978; 75(8):3737-41. PMC: 392861. DOI: 10.1073/pnas.75.8.3737. View

20.

Mandel M, Higa A . Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970; 53(1):159-62. DOI: 10.1016/0022-2836(70)90051-3. View