Escherichia Coli Mutants Defective in the UncH Gene

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Jan 1

PMID 6294057

Citations 25

Authors

R HUMBERT

W S Brusilow

R P Gunsalus

D J Klionsky

R D Simoni

Affiliations

Soon will be listed here.

Abstract

Plasmids carrying cloned segments of the unc operon of Escherichia coli have been used in genetic complementation analyses to identify three independent mutants defective in the uncH gene, which codes for the delta subunit of the ATP synthetase. Mutations in other unc genes have also been mapped by this technique. ATPase activity was present in extracts of the uncH mutants, but the enzyme was not as tightly bound to the membrane as it was in the parental strain. ATP-dependent membrane energization was absent in membranes isolated from the uncH mutants and could not be restored by adding normal F1 ATPase from the wild-type strain. F1 ATPase prepared from uncH mutants could not restore ATP-dependent membrane energization when added to wild-type membranes depleted of F1. Membranes of the uncH mutants were not rendered proton permeable as a result of washing with low-ionic-strength buffer.

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References

Simoni R, Shallenberger M . Coupling of energy to active transport of amino acids in Escherichia coli. Proc Natl Acad Sci U S A. 1972; 69(9):2663-7. PMC: 427011. DOI: 10.1073/pnas.69.9.2663. View

Smith J, Sternweis P . Purification of membrane attachment and inhibitory subunits of the proton translocating adenosine triphosphatase from Escherichia coli. Biochemistry. 1977; 16(2):306-11. DOI: 10.1021/bi00621a023. View

Nieuwenhuis F, Kanner B, Gutnick D, Postma P, van Dam K . Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation. Biochim Biophys Acta. 1973; 325(1):62-71. DOI: 10.1016/0005-2728(73)90151-5. View

Kanner B, Gutnick D . Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli. J Bacteriol. 1972; 111(1):287-9. PMC: 251270. DOI: 10.1128/jb.111.1.287-289.1972. View

Futai M, Sternweis P, HEPPEL L . Purification and properties of reconstitutively active and inactive adenosinetriphosphatase from Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(7):2725-9. PMC: 388541. DOI: 10.1073/pnas.71.7.2725. View

Gibson F, Cox G, Downie J, Radik J . Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation. Biochem J. 1977; 162(3):665-70. PMC: 1164651. DOI: 10.1042/bj1620665. View

Cox G, Downie J, Gibson F, Radik J . Genetic complementation between two mutant unc alleles (unc A401 and unc D409) affecting the Fl portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. Biochem J. 1978; 170(3):593-8. PMC: 1183936. DOI: 10.1042/bj1700593. View

Gibson F, Downie J, Cox G, Radik J . Mu-induced polarity in the unc operon of Escherichia coli. J Bacteriol. 1978; 134(3):728-36. PMC: 222317. DOI: 10.1128/jb.134.3.728-736.1978. View

Downie J, Senior A, Gibson F, Cox G . A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli. J Bacteriol. 1979; 137(2):711-8. PMC: 218347. DOI: 10.1128/jb.137.2.711-718.1979. View

10.

Kanazawa H, Miki T, Tamura F, Yura T, Futai M . Specialized transducing phage lambda carrying the genes for coupling factor of oxidative phosphorylation of Escherichia coli: increased synthesis of coupling factor on induction of prophage lambda asn. Proc Natl Acad Sci U S A. 1979; 76(3):1126-30. PMC: 383202. DOI: 10.1073/pnas.76.3.1126. View

11.

Downie J, Gibson F, Cox G . Membrane adenosine triphosphatases of prokaryotic cells. Annu Rev Biochem. 1979; 48:103-31. DOI: 10.1146/annurev.bi.48.070179.000535. View

12.

Gunsalus R, Zurawski G, Yanofsky C . Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome. J Bacteriol. 1979; 140(1):106-13. PMC: 216785. DOI: 10.1128/jb.140.1.106-113.1979. View

13.

HUMBERT R, Simoni R . Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli. J Bacteriol. 1980; 142(1):212-20. PMC: 293932. DOI: 10.1128/jb.142.1.212-220.1980. View

14.

Downie J, Langman L, Cox G, Yanofsky C, Gibson F . Subunits of the adenosine triphosphatase complex translated in vitro from the Escherichia coli unc operon. J Bacteriol. 1980; 143(1):8-17. PMC: 294171. DOI: 10.1128/jb.143.1.8-17.1980. View

15.

Bachmann B, Low K . Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980; 44(1):1-56. PMC: 373233. DOI: 10.1128/mr.44.1.1-56.1980. View

16.

Downie J, Cox G, Langman L, Ash G, Becker M, Gibson F . Three genes coding for subunits of the membrane sector (F0) of the Escherichia coli adenosine triphosphatase complex. J Bacteriol. 1981; 145(1):200-10. PMC: 217262. DOI: 10.1128/jb.145.1.200-210.1981. View

17.

Brusilow W, Gunsalus R, Hardeman E, Decker K, Simoni R . In vitro synthesis of the F0 and F1 components of the proton translocating ATPase of Escherichia coli. J Biol Chem. 1981; 256(7):3141-4. View

18.

Hanson R, Kennedy E . Energy-transducing adenosine triphosphatase from Escherichia coli: purification, properties, and inhibition by antibody. J Bacteriol. 1973; 114(2):772-81. PMC: 251838. DOI: 10.1128/jb.114.2.772-781.1973. View

19.

Kanazawa H, Mabuchi K, Kayano T, Tamura F, Futai M . Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli. Biochem Biophys Res Commun. 1981; 100(1):219-25. DOI: 10.1016/s0006-291x(81)80085-x. View

20.

Cox G, Downie J, Langman L, Senior A, Ash G, Fayle D . Assembly of the adenosine triphosphatase complex in Escherichia coli: assembly of F0 is dependent on the formation of specific F1 subunits. J Bacteriol. 1981; 148(1):30-42. PMC: 216163. DOI: 10.1128/jb.148.1.30-42.1981. View