Mu-induced Polarity in the Unc Operon of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Jun 1

PMID 149112

Citations 41

Authors

F Gibson

J A Downie

G B Cox

J Radik

Affiliations

Soon will be listed here.

Abstract

Mutant strains of Escherichia coli were isolated in which mutator (Mu) phage was inserted into various unc genes. Partial diploid strains were prepared from each of the Mu-induced unc mutants by using F-plasmids carrying mutations in one of the known unc genes (uncA, uncB, uncC, or uncD). The partial diploid strains and the corresponding segregant strains were examined for their ability to grow on succinate. The aerobic growth yields on limiting concentrations of glucose were also determined. Magnesium-stimulated adenosine triphosphatase activities, ATP-dependent transhydrogenase activities, and Atebrin fluorescence quenching activities were determined by using membrane preparations from each strain. Genetic complementation was assessed from the results obtained, and it was concluded that the four unc genes examined are part of a single transcriptional unit and that they are transcribed in the order uncBADC.

Citing Articles

Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase.

Howe C, Auffret A, Doherty A, Bowman C, Dyer T, Gray J Proc Natl Acad Sci U S A. 1982; 79(22):6903-7.

PMID: 16593250 PMC: 347242. DOI: 10.1073/pnas.79.22.6903.

Membrane integration and function of the three F0 subunits of the ATP synthase of Escherichia coli K12.

Friedl P, Hoppe J, Gunsalus R, Michelsen O, von Meyenburg K, Schairer H EMBO J. 1983; 2(1):99-103.

PMID: 11894918 PMC: 555094. DOI: 10.1002/j.1460-2075.1983.tb01388.x.

Energy-driven subunit rotation at the interface between subunit a and the c oligomer in the F(O) sector of Escherichia coli ATP synthase.

Hutcheon M, Duncan T, Ngai H, CROSS R Proc Natl Acad Sci U S A. 2001; 98(15):8519-24.

PMID: 11438702 PMC: 37468. DOI: 10.1073/pnas.151236798.

Subunit rotation in Escherichia coli FoF1-ATP synthase during oxidative phosphorylation.

Zhou Y, Duncan T, CROSS R Proc Natl Acad Sci U S A. 1997; 94(20):10583-7.

PMID: 9380678 PMC: 23410. DOI: 10.1073/pnas.94.20.10583.

Limited differential mRNA inactivation in the atp (unc) operon of Escherichia coli.

Lagoni O, von Meyenburg K, Michelsen O J Bacteriol. 1993; 175(18):5791-7.

PMID: 7690747 PMC: 206657. DOI: 10.1128/jb.175.18.5791-5797.1993.

References

Novick R, CLOWES R, Cohen S, Curtiss 3rd R, Datta N, Falkow S . Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976; 40(1):168-89. PMC: 413948. DOI: 10.1128/br.40.1.168-189.1976. View

Bachmann B, Low K, Taylor A . Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976; 40(1):116-67. PMC: 413944. DOI: 10.1128/br.40.1.116-167.1976. View

Haddock B, Jones C . Bacterial respiration. Bacteriol Rev. 1977; 41(1):47-99. PMC: 413996. DOI: 10.1128/br.41.1.47-99.1977. View

Taylor A . BACTERIOPHAGE-INDUCED MUTATION IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963; 50:1043-51. PMC: 221271. DOI: 10.1073/pnas.50.6.1043. View

Gibson F, Cox G, Downie J, Radik J . Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation. Biochem J. 1977; 162(3):665-70. PMC: 1164651. DOI: 10.1042/bj1620665. View

Gibson F, Cox G, Downie J, Radik J . A mutation affecting a second component of the F0 portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. The uncC424 allele. Biochem J. 1977; 164(1):193-8. PMC: 1164774. DOI: 10.1042/bj1640193. View

Cox G, Downie J, Gibson F, Radik J . Genetic complementation between two mutant unc alleles (unc A401 and unc D409) affecting the Fl portion of the magnesium ion-stimulated adenosine triphosphatase of Escherichia coli K12. Biochem J. 1978; 170(3):593-8. PMC: 1183936. DOI: 10.1042/bj1700593. View

Simoni R, Postma P . The energetics of bacterial active transport. Annu Rev Biochem. 1975; 44:523-54. DOI: 10.1146/annurev.bi.44.070175.002515. View

Butlin J, Cox G, Gibson F . Oxidative phosphorylation in Escherichia coli K-12: the genetic and biochemical characterisations of a strain carrying a mutation in the uncB gene. Biochim Biophys Acta. 1973; 292(2):366-75. DOI: 10.1016/0005-2728(73)90043-1. View

10.

Nieuwenhuis F, Kanner B, Gutnick D, Postma P, van Dam K . Energy conservation in membranes of mutants of Escherichia coli defective in oxidative phosphorylation. Biochim Biophys Acta. 1973; 325(1):62-71. DOI: 10.1016/0005-2728(73)90151-5. View

11.

Cox G, Gibson F . Studies on electron transport and energy-linked reactions using mutants of Escherichia coli. Biochim Biophys Acta. 1974; 346(1):1-25. DOI: 10.1016/0304-4173(74)90010-x. View

12.

Cox G, Newton N, Gibson F, SNOSWELL A, Hamilton J . The function of ubiquinone in Escherichia coli. Biochem J. 1970; 117(3):551-62. PMC: 1178960. DOI: 10.1042/bj1170551. View

13.

Butlin J, Cox G, Gibson F . Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1971; 124(1):75-81. PMC: 1177115. DOI: 10.1042/bj1240075. View

14.

Cox G, Gibson F, McCann L, Butlin J, Crane F . Reconstitution of the energy-linked transhydrogenase activity in membranes from a mutant strain of Escherichia coli K12 lacking magnesium ion- or calcium ion-stimulated adenosine triphosphatase. Biochem J. 1973; 132(4):689-95. PMC: 1177644. DOI: 10.1042/bj1320689. View

15.

Cox G, Newton N, Butlin J, Gibson F . The energy-linked transhydrogenase reaction in respiratory mutants of Escherichia coli K12. Biochem J. 1971; 125(2):489-93. PMC: 1178083. DOI: 10.1042/bj1250489. View

16.

Haddock B, Downie J . The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence. Biochem J. 1974; 142(3):703-6. PMC: 1168339. DOI: 10.1042/bj1420703. View

17.

Daniell E, Roberts R, Abelson J . Mutations in the lactose operon caused by bacteriophage Mu. J Mol Biol. 1972; 69(1):1-8. DOI: 10.1016/0022-2836(72)90019-8. View

18.

Daniell E, Abelson J . Lac messenger RNA in lac Z gene mutants of Escherichia coli caused by insertion of bacteriophage Mu. J Mol Biol. 1973; 76(2):319-22. DOI: 10.1016/0022-2836(73)90395-1. View

19.

Deamer D, Prince R, Crofts A . The response of fluorescent amines to pH gradients across liposome membranes. Biochim Biophys Acta. 1972; 274(2):323-35. DOI: 10.1016/0005-2736(72)90180-0. View

20.

Luria S, BURROUS J . Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957; 74(4):461-76. PMC: 289941. DOI: 10.1128/jb.74.4.461-476.1957. View