Cointegrate Formation Between Homologous Plasmids in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Sep 1

PMID 6286589

Citations 17

Authors

B C Peterson

H Hashimoto

R H Rownd

Affiliations

Soon will be listed here.

Abstract

Conjugation experiments were performed in which the donor was Escherichia coli K-12 strain KP245 containing either R plasmid NR1 plus an ampicillin-resistant derivative of ColE1 (*ColE1::Tn3, called RSF2124) or NR1 plus RSF2124 carrying a cloned EcoRI fragment of NR1. The recipient was the polA amber mutant JG112, in which RSF2124 cannot replicate. Ampicillin-resistant transconjugants can arise only when the genes for ampicillin resistance are linked to NR1 or are transposed to the host chromosome. When EcoRI fragment A of NR1 (20.5 kilobases) was cloned to RSF2124, the frequency of cotransfer of ampicillin resistance with tetracycline resistance was 25 to 60%. Plasmid DNA from these ampicillin-resistant transconjugant cells was analyzed by gel electrophoresis and was shown to be a cointegrate of NR1 and the RSF2124 derivative. Analysis of plasmid DNA isolated from donor cultures showed that the cointegrates were present before conjugation, which indicates that the mating does not stimulate cointegrate formation. When the cloned fragment was EcoRI fragment H of NR1 (4.8 kilobases), the frequency of cotransfer of ampicillin resistance with tetracycline resistance was about 4%, and the majority of the ampicillin-resistant transconjugants were found to contain cointegrate plasmids. When the donor contained NR1 and RSF2124, the frequency of cotransfer of ampicillin resistance was less than 0.1%, and analysis of plasmid DNA from the ampicillin-resistant transconjugants showed that Tn3 had been transposed onto NR1. These data suggest that plasmids which share homology may exist in cointegrate form to a high degree within a host cell.

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References

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

Yokota T, Kanamaru Y, Mori R, Akiba T . Recombination between a thermosensitive kanamycin resistance factor and a nonthermosensitive multiple-drug resistant factor. J Bacteriol. 1969; 98(3):863-73. PMC: 315265. DOI: 10.1128/jb.98.3.863-873.1969. View

Nakaya R, Rownd R . Transduction of R factors by a Proteus mirabilis bacteriophage. J Bacteriol. 1971; 106(3):773-83. PMC: 248691. DOI: 10.1128/jb.106.3.773-783.1971. View

Finnegan D, Willetts N . Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Mol Gen Genet. 1971; 111(3):256-64. DOI: 10.1007/BF00433110. View

Finnegan D, Willetts N . The nature of the transfer inhibitor of several F-like plasmids. Mol Gen Genet. 1972; 119(1):57-66. DOI: 10.1007/BF00270444. View

Goldfarb D, Goldberg G, Chernin L, Gukova L, Avdienko I, Kuznetsova B . A protein produced by male strains of Escherichia coli K-12 which increases the yield of recombinants in conjugation: its nature and mode of action. Mol Gen Genet. 1973; 120(3):211-26. DOI: 10.1007/BF00267153. View

Kingsbury D, Helinski D . Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973; 114(3):1116-24. PMC: 285372. DOI: 10.1128/jb.114.3.1116-1124.1973. View

Goldfarb D, Gukova L, Chernin L, Avdienko I, Mnatsakanian G, Kushner I . Rsf mutants of Escherichia coli HfrC defective in the production of the factor stimulating recombination in conjugation. Mol Gen Genet. 1974; 129(4):295-310. DOI: 10.1007/BF00265694. View

LEDERBERG E, Cohen S . Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974; 119(3):1072-4. PMC: 245717. DOI: 10.1128/jb.119.3.1072-1074.1974. View

10.

Helling R, Goodman H, Boyer H . Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974; 14(5):1235-44. PMC: 355640. DOI: 10.1128/JVI.14.5.1235-1244.1974. View

11.

Lovett M, Helinski D . Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K. Biochemistry. 1974; 13(27):5484-90. DOI: 10.1021/bi00724a005. View

12.

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

13.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

14.

So M, Gill R, Falkow S . The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet. 1975; 142(3):239-49. DOI: 10.1007/BF00425649. View

15.

Tanak N, Cramer J, Rownd R . EcoRI restriction endonuclease map of the composite R plasmid NR1. J Bacteriol. 1976; 127(1):619-36. PMC: 233095. DOI: 10.1128/jb.127.1.619-636.1976. View

16.

Manis J, Whitfield H . Physical characterization of a plasmid cointegrate containing an F'his gnd element and the Salmonella typhimurium LT2 cryptic plasmid. J Bacteriol. 1977; 129(3):1601-6. PMC: 235140. DOI: 10.1128/jb.129.3.1601-1606.1977. View

17.

Womble D, Taylor D, Rownd R . Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation. J Bacteriol. 1977; 130(1):148-53. PMC: 235186. DOI: 10.1128/jb.130.1.148-153.1977. View

18.

Miki T, Easton A, Rownd R . Mapping of the resistance genes of the R plasmid NR1. Mol Gen Genet. 1978; 158(3):217-24. DOI: 10.1007/BF00267192. View

19.

Timmis K, Andres I, Achtman M . Fertility repression of F-like conjugative plasmids: physical mapping of the R6--5 finO and finP cistrons and identification of the finO protein. Proc Natl Acad Sci U S A. 1978; 75(12):5836-40. PMC: 393070. DOI: 10.1073/pnas.75.12.5836. View

20.

Miller J, MANIS J, Kline B, Bishop A . Nonintegrated plasmid-folded chromosome complexes: genetic studies on formation and possible relationship to plasmid replication. Plasmid. 1978; 1(3):273-83. DOI: 10.1016/0147-619x(78)90045-8. View