Fertility Inhibition Gene of Plasmid R100

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Mar 11

PMID 2951652

Citations 7

Authors

S A McIntire

W B Dempsey

Affiliations

Soon will be listed here.

Abstract

The fin0 gene of R100 was isolated from the Fin0+ transducing phage VA lambda 57. The limits of the gene were determined by BAL31 digestions and by analysis of deletion mutations derived from an internal restriction site. The DNA sequence contained an open reading frame of 558 nucleotides that would encode a protein of 21,268 daltons. Synthesis of such a protein was observed only when the fragment was cloned in front of the TAC promoter. Deletions entering the large open reading frame from either end were Fin0-, while internal frame shift mutations retained high Fin0 activity. One such strain had a 13 bp internal deletion that would produce a protein of 63 amino acid residues of which 21 were basic. We were consequently unable to rigorously establish that the 558 base orf encoded a fin0 product. The strand opposite the large open reading frame contained several transcription termination signals, and it is possible that the active gene product is one or two small RNAs from this strand.

Citing Articles

Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation.

Anthony K, Klimke W, Manchak J, Frost L J Bacteriol. 1999; 181(17):5149-59.

PMID: 10464182 PMC: 94017. DOI: 10.1128/JB.181.17.5149-5159.1999.

Mosaic structure of plasmids from natural populations of Escherichia coli.

Boyd E, HILL C, Rich S, Hartl D Genetics. 1996; 143(3):1091-100.

PMID: 8807284 PMC: 1207381. DOI: 10.1093/genetics/143.3.1091.

Analysis of the sequence and gene products of the transfer region of the F sex factor.

Frost L, Skurray R Microbiol Rev. 1994; 58(2):162-210.

PMID: 7915817 PMC: 372961. DOI: 10.1128/mr.58.2.162-210.1994.

oriT sequence of the antibiotic resistance plasmid R100.

McIntire S, Dempsey W J Bacteriol. 1987; 169(8):3829-32.

PMID: 3611030 PMC: 212476. DOI: 10.1128/jb.169.8.3829-3832.1987.

Transcript analysis of the plasmid R100 traJ and finP genes.

Dempsey W Mol Gen Genet. 1987; 209(3):533-44.

PMID: 3323829 DOI: 10.1007/BF00331160.

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