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Active Genes Are Sensitive to Deoxyribonuclease I During Metaphase

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Journal Science
Specialty Science
Date 1982 Aug 13
PMID 6283640
Citations 30
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Abstract

The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.

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