Restriction Enzyme Banding of Mouse Metaphase Chromosomes

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1984 Jan 1

PMID 6090079

Citations 14

Authors

M Kaelbling

D A Miller

O J MILLER

Affiliations

Soon will be listed here.

Abstract

Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Alu I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.

Citing Articles

Heterochromatin heterogeneity in Hypostomus prope unae (Steindachner, 1878) (Siluriformes, Loricariidae)from Northeastern Brazil.

Bitencourt J, Affonso P, Giuliano-Caetano L, Dias A Comp Cytogenet. 2013; 5(4):329-44.

PMID: 24260639 PMC: 3833786. DOI: 10.3897/CompCytogen.v5i4.1149.

Inactive X chromosome has the highest concentration of unmethylated Hha I sites.

Viegas-Pequignot E, Dutrillaux B, Thomas G Proc Natl Acad Sci U S A. 1988; 85(20):7657-60.

PMID: 3262875 PMC: 282251. DOI: 10.1073/pnas.85.20.7657.

The Alu I-induced bands in metaphase chromosomes of orangutan (Pongo pygmaeus). Implications for the distribution pattern of highly repetitive DNA sequences.

De Stefano G, Romano E, Ferrucci L Hum Genet. 1986; 72(3):268-71.

PMID: 3007330 DOI: 10.1007/BF00291894.

Chromosome banding in Amphibia. XII. Restriction endonuclease banding.

Schmid M, de Almeida C Chromosoma. 1988; 96(4):283-90.

PMID: 2838228 DOI: 10.1007/BF00286915.

Restriction endonuclease resistant chromatin in human chromosomes.

Babu A, Verma R Mol Gen Genet. 1988; 211(2):367-8.

PMID: 2832707 DOI: 10.1007/BF00330618.

References

Southern E . Long range periodicities in mouse satellite DNA. J Mol Biol. 1975; 94(1):51-69. DOI: 10.1016/0022-2836(75)90404-0. View

Arnheim N, Treco D, Taylor B, Eicher E . Distribution of ribosomal gene length variants among mouse chromosomes. Proc Natl Acad Sci U S A. 1982; 79(15):4677-80. PMC: 346739. DOI: 10.1073/pnas.79.15.4677. View

Miller D, Choi Y, MILLER O . Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes. Science. 1983; 219(4583):395-7. DOI: 10.1126/science.6294832. View

Cooke H, McKay R . Evolution of a human Y chromosome-specific repeated sequence. Cell. 1978; 13(3):453-60. DOI: 10.1016/0092-8674(78)90319-7. View

LIMA-DE-FARIA A, Isaksson M, Olsson E . Action of restriction endonucleases on the DNA and chromosomes of Muntiacus muntjak. Hereditas. 1980; 92(2):267-73. DOI: 10.1111/j.1601-5223.1980.tb01707.x. View

Comings D . Mechanisms of chromosome banding and implications for chromosome structure. Annu Rev Genet. 1978; 12:25-46. DOI: 10.1146/annurev.ge.12.120178.000325. View

Pietras D, Bennett K, Siracusa L, Chapman V, Gross K, Hastie N . Construction of a small Mus musculus repetitive DNA library: identification of a new satellite sequence in Mus musculus. Nucleic Acids Res. 1983; 11(20):6965-83. PMC: 326432. DOI: 10.1093/nar/11.20.6965. View

Lipchitz L, Axel R . Restriction endonuclease cleavage of satellite DNA in intact bovine nuclei. Cell. 1976; 9(2):355-64. DOI: 10.1016/0092-8674(76)90125-2. View

Gazit B, Cedar H, Lerer I, Voss R . Active genes are sensitive to deoxyribonuclease I during metaphase. Science. 1982; 217(4560):648-50. DOI: 10.1126/science.6283640. View

10.

Lica L, Hamkalo B . Preparation of centromeric heterochromatin by restriction endonuclease digestion of mouse L929 cells. Chromosoma. 1983; 88(1):42-9. DOI: 10.1007/BF00329502. View

11.

Beauchamp R, Mitchell A, Buckland R, Bostock C . Specific arrangements of human satellite III DNA sequences in human chromosomes. Chromosoma. 1979; 71(2):153-66. DOI: 10.1007/BF00292820. View

12.

Sahasrabuddhe C, Pathak S, Hsu T . Responses of mammalian metaphase chromosomes to endonuclease digestion. Chromosoma. 1978; 69(3):331-8. DOI: 10.1007/BF00332136. View

13.

Pardue M, Gall J . Chromosomal localization of mouse satellite DNA. Science. 1970; 168(3937):1356-8. DOI: 10.1126/science.168.3937.1356. View

14.

Matsukuma S, UTAKOJI T . Uneven extraction of protein in Chinese hamster chromosomes during G-staining procedures. Exp Cell Res. 1976; 97(2):297-303. DOI: 10.1016/0014-4827(76)90620-0. View

15.

Brown S, Dover G . The specific organisation of satellite DNA sequences on the X-chromosome of Mus musculus: partial independence of chromosome evolution. Nucleic Acids Res. 1980; 8(4):781-92. PMC: 327310. View

16.

Mezzanotte R, Bianchi U, Vanni R, Ferrucci L . Chromatin organization and restriction endonuclease activity on human metaphase chromosomes. Cytogenet Cell Genet. 1983; 36(3):562-6. DOI: 10.1159/000131973. View

17.

Kerem B, Goitein R, Richler C, Marcus M, Cedar H . In situ nick-translation distinguishes between active and inactive X chromosomes. Nature. 1983; 304(5921):88-90. DOI: 10.1038/304088a0. View

18.

Horz W, Zachau H . Characterization of distinct segments in mouse satellite DNA by restriction nucleases. Eur J Biochem. 1977; 73(2):383-92. DOI: 10.1111/j.1432-1033.1977.tb11329.x. View

19.

Miller D, Tantravahi R, Dev V, MILLER O . Q- and C-band chromosome markers in inbred strains of Mus musculus. Genetics. 1976; 84(1):67-75. PMC: 1213566. DOI: 10.1093/genetics/84.1.67. View

20.

Dev V, Miller D, MILLER O, Marshall Jr J, Hsu T . Quinacrine fluorescence of Mus cervicolor chromosomes. Bright centrometric heterochromatin. Exp Cell Res. 1973; 79(2):475-9. DOI: 10.1016/0014-4827(73)90475-8. View