Non Random Distribution of Lesions Induced by Deoxyribonuclease I in Human Chromosomes

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Feb 24

PMID 22358435

Authors

F Nuzzo

A Casati

E Raimondi

Affiliations

Soon will be listed here.

Abstract

We studied the action of deoxyribonuclease I on human lymphocytes in order to determine the localization of the DNAase induced aberrations. Our results indicate a non-random distribution of the lesions on chromosome regions which may reflect a differential pattern of sensitivity to the enzyme. Furthermore we observed a correspondence between the preferential DNAase induced breaks and fragile sites that are expressed in lymphocytes maintained in medium without folic acid. A possible interpretation of our findings is that the accessibility to DNAase and/or the efficiency of the repair systems depend on the chromatin structure that influences also the expression of some common fragile sites.

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