Expression of the Herpes Simplex Virus Thymidine Kinase Gene in Saccharomyces Cerevisiae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6278252

Citations 34

Authors

J B McNeil

J D Friesen

Affiliations

Soon will be listed here.

Abstract

Yeast plasmids have been constructed that carry the Herpes simplex Virus type 1 (HSV-1) thymidine kinase (TK) gene which is functionally expressed in Saccharomyces cerevisiae. The expression of the TK gene appears to be due to transcriptional read-through from a yeast promoter that lies on the 3' side of the HIS3 gene. The TK+ yeast possesses in vitro thymidine kinase activity which is absent in the original yeast strain. Yeast strains auxotrophic for thymidine monophosphate (dTMP) (tmp1) can grown on thymidine-containing medium after transformation with these plasmids. Tmp+, TK+ S. cerevisiae whose de novo synthesis of dTMP is inhibited with amethopterin plus sufanilamide is also capable of growth in thymidine. S. cerevisiae transformed with such plasmids is capable of incorporating thymidine and bromodeoxyuridine into DNA.

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References

Fath W, Brendel M . Specific DNA-labelling by exogenous thymidine-5'-monophosphate in Saccharomyces cerevisiae. Mol Gen Genet. 1974; 131(1):57-67. DOI: 10.1007/BF00269387. View

Laskowski W . Mutants of Saccharomyces able to grow after inhibition of thymidine phosphate synthesis. Mol Gen Genet. 1973; 125(3):275-7. DOI: 10.1007/BF00270749. View

Kunz B, Barclay B, Game J, Little J, HAYNES R . Induction of mitotic recombination in yeast by starvation for thymine nucleotides. Proc Natl Acad Sci U S A. 1980; 77(10):6057-61. PMC: 350212. DOI: 10.1073/pnas.77.10.6057. View

McNeil J, Storms R, Friesen J . High frequency recombination and the expression of genes cloned on chimeric yeast plasmids: Identification of a fragment of 2-μm circle essential for transformation. Curr Genet. 2013; 2(1):17-251. DOI: 10.1007/BF00445690. View

McKnight S, Gavis E . Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro. Nucleic Acids Res. 1980; 8(24):5931-48. PMC: 328063. DOI: 10.1093/nar/8.24.5931. View

Beggs J, van den Berg J, Ooyen A, Weissmann C . Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae. Nature. 1980; 283(5750):835-40. DOI: 10.1038/283835a0. View

Grivell A, Jackson J . Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. J Gen Microbiol. 1968; 54(2):307-17. DOI: 10.1099/00221287-54-2-307. View

Collins J, Hohn B . Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc Natl Acad Sci U S A. 1978; 75(9):4242-6. PMC: 336088. DOI: 10.1073/pnas.75.9.4242. View

Little J, HAYNES R . Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate. Mol Gen Genet. 1979; 168(2):141-51. DOI: 10.1007/BF00431440. View

10.

Clewell D, Helinski D . Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970; 9(22):4428-40. DOI: 10.1021/bi00824a026. View

11.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

12.

Mercereau-Puijalon O, Lacroute F, Kourilsky P . Synthesis of a chicken ovalbumin-like protein in the yeast Saccharomyces cerevisiae. Gene. 1980; 11(1-2):163-7. DOI: 10.1016/0378-1119(80)90096-7. View

13.

Cohen J, Eccleshall T, Needleman R, Federoff H, Buchferer B, MARMUR J . Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase. Proc Natl Acad Sci U S A. 1980; 77(2):1078-82. PMC: 348427. DOI: 10.1073/pnas.77.2.1078. View

14.

Enquist L, Woude G, Wagner M, SMILEY J, Summers W . Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus. Gene. 1979; 7(3-4):335-42. DOI: 10.1016/0378-1119(79)90052-0. View

15.

Hinnen A, Hicks J, Fink G . Transformation of yeast. Proc Natl Acad Sci U S A. 1978; 75(4):1929-33. PMC: 392455. DOI: 10.1073/pnas.75.4.1929. View

16.

Lennox E . Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955; 1(2):190-206. DOI: 10.1016/0042-6822(55)90016-7. View

17.

Storms R, McNeil J, Khandekar P, An G, Parker J, Friesen J . Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae. J Bacteriol. 1979; 140(1):73-82. PMC: 216781. DOI: 10.1128/jb.140.1.73-82.1979. View

18.

Wickner R . Mutants of Saccharomyces cerevisiae that incorporate deoxythymidine-5'-monophosphate into deoxyribonucleic acid in vivo. J Bacteriol. 1974; 117(1):252-60. PMC: 246551. DOI: 10.1128/jb.117.1.252-260.1974. View

19.

KLEMPERER H, Haynes G, Shedden W, Watson D . A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus. Virology. 1967; 31(1):120-8. DOI: 10.1016/0042-6822(67)90015-3. View

20.

Henikoff S, Tatchell K, Hall B, Nasmyth K . Isolation of a gene from Drosophila by complementation in yeast. Nature. 1981; 289(5793):33-7. DOI: 10.1038/289033a0. View