Isolation and Characterization of Yeast Mutants Auxotrophic for 2'-deoxythymidine 5'-monophosphate

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1979 Jan 10

PMID 377008

Citations 29

Authors

J G Little

R H HAYNES

Affiliations

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Abstract

Mutant strains of Saccharomyces cerevisiae auxotrophic for deoxythymidine monophosphate (dTMP) were isolated and characterized. Two distinct classes of auxotrophs were obtained. One class had a simple requirement for dTMP and was analogous to thymine-requiring bacteria. The second class required dTMP, adenine, histidine and methionine and this complex nutritional phenotype was due to defects in folate metabolism. The dTMP-dependent growth of respiratory-competent grande auxotrophs was found to be markedly affected by media composition and carbon source. In the absence of dTMP thymineless death occurred in both mutant classes.

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