Characterization of the Asialoglycoprotein Receptor in a Continuous Hepatoma Line

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1981 Sep 10

PMID 6267054

Citations 56

Authors

A L Schwartz

S E Fridovich

B B Knowles

H F Lodish

Affiliations

Soon will be listed here.

Abstract

The asialoglycoprotein receptor has been identified on a continuous human hepatoma cell line, HepG2. This receptor requires Ca2+ for ligand binding and is specific for asialoglycoprotein. There are approximately 150,000 ligand molecules bound/cell at 4 degrees C. These receptors represent a homogeneous population of high affinity binding sites with Kd = 7 X 10(-9) M. From the rate of 125I-ASOR binding at 4 degrees C, kon was 0.95 X 10(6) M-1 min-1. Uptake of 125I-ASOR at 37 degrees C was approximately 0.02 pmol/min/10(6) cells.

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