Gene Expression: Chemical Synthesis and Molecular Cloning of a Bacteriophage T5 (T5P25) Early Promoter

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Sep 10

PMID 6310520

Citations 8

Authors

J Rommens

D MacKnight

E Jay

Affiliations

Soon will be listed here.

Abstract

A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototype for an approach to the study of the in vivo structure-function relationships and efficiency of promoters.

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References

Clewell D, Helinski D . Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969; 62(4):1159-66. PMC: 223628. DOI: 10.1073/pnas.62.4.1159. View

HAWLEY D, McClure W . Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983; 11(8):2237-55. PMC: 325881. DOI: 10.1093/nar/11.8.2237. View

Schaller H, Gray C, Herrmann K . Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. Proc Natl Acad Sci U S A. 1975; 72(2):737-41. PMC: 432391. DOI: 10.1073/pnas.72.2.737. View

Shaw W . Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975; 43:737-55. DOI: 10.1016/0076-6879(75)43141-x. View

Dugaiczyk A, Boyer H, Goodman H . Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975; 96(1):171-84. DOI: 10.1016/0022-2836(75)90189-8. View

Maniatis T, Jeffrey A, van deSande H . Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975; 14(17):3787-94. DOI: 10.1021/bi00688a010. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Seeburg P, Nusslein C, Schaller H . Interaction of RNA polymerase with promoters from bacteriophage fd. Eur J Biochem. 1977; 74(1):107-13. DOI: 10.1111/j.1432-1033.1977.tb11372.x. View

von Gabain A, Bujard H . Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity. Mol Gen Genet. 1977; 157(3):301-11. DOI: 10.1007/BF00268667. View

10.

Johnsrud L . Contacts between Escherichia coli RNA polymerase and a lac operon promoter. Proc Natl Acad Sci U S A. 1978; 75(11):5314-8. PMC: 392953. DOI: 10.1073/pnas.75.11.5314. View

11.

von Gabain A, Bujard H . Interaction of Escherichia coli RNA polymerase with promoters of several coliphage and plasmid DNAs. Proc Natl Acad Sci U S A. 1979; 76(1):189-93. PMC: 382902. DOI: 10.1073/pnas.76.1.189. View

12.

Rodriguez R, West R, Heyneker H, Bolivar F, Boyer H . Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. Nucleic Acids Res. 1979; 6(10):3267-87. PMC: 327933. DOI: 10.1093/nar/6.10.3267. View

13.

Tu C, Cohen S . Translocation specificity of the Tn3 element: characterization of sites of multiple insertions. Cell. 1980; 19(1):151-60. DOI: 10.1016/0092-8674(80)90396-7. View

14.

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

15.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

16.

Siebenlist U, Simpson R, Gilbert W . E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980; 20(2):269-81. DOI: 10.1016/0092-8674(80)90613-3. View

17.

Winberg G, Hammarskjold M . Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA. Nucleic Acids Res. 1980; 8(2):253-64. PMC: 327265. DOI: 10.1093/nar/8.2.253. View

18.

Gait M, Singh M, SHEPPARD R, Edge M, Greene A, Heathcliffe G . Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates. Nucleic Acids Res. 1980; 8(5):1081-96. PMC: 323974. DOI: 10.1093/nar/8.5.1081. View

19.

Miyoshi K, Miyake T, Hozumi T, Itakura K . Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method. Nucleic Acids Res. 1980; 8(22):5473-89. PMC: 324315. DOI: 10.1093/nar/8.22.5473. View

20.

Dobrynin V, Korobko V, Severtsova I, Bystrov N, Chuvpilo S, KOLOSOV M . Synthesis of a model promoter for gene expression in Escherichia coli. Nucleic Acids Symp Ser. 1980; (7):365-76. View