Induction of Idiotype-specific Suppressor T Cells with Antigen/antibody Complexes

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1983 Jun 1

PMID 6222133

Citations 7

Authors

M J Caulfield

K J Luce

M R Proffitt

J cerny

Affiliations

Soon will be listed here.

Abstract

The effects of immune complexes on the antibody response of BALB/c mice to Streptococcus pneumoniae R36a (Pn) were investigated. The cell wall polysaccharide (PnC) extracted from Pn was used to form complexes with TEPC-15, a myeloma protein that binds to phosphorylcholine determinants on the PnC. Complexes formed at equivalence were cultured with splenic T cells from BALB/c mice for 2 d, and then the cells were added to fresh BALB/c spleen cell cultures to test their effect on the antibody response to Pn, a response dominated by the T15 idiotype family. The results indicate that TEPC-15/PnC complexes induced potent suppressor T cells (Ts) whereas cells cultured with free antigen or free antibody alone had no effect on the plaque-forming cell response to Pn. The suppression was specific since the response to control antigens such as sheep erythrocytes was unaffected. The suppression appears to be idiotype-specific since the Ts had a relatively weak (and in some cases no) effect on the anti-Pn response of BALB/c mice that had been suppressed for T15 idiotopes by neonatal injection of a monoclonal anti-T15 antibody, MaId 5-4. Furthermore, cells cultured with TEPC-15/PnC complexes were shown to express specific receptors for TEPC-15 idiotopes. The results indicate that antigen/antibody complexes may have important immunoregulatory effects because they are potent inducers of idiotype-specific Ts.

Citing Articles

T-cell dependent response to immune complexes abrogates B-cell unresponsiveness to pneumococcal cell wall polysaccharide.

Caulfield M, Stanko D Immunology. 1995; 86(3):331-5.

PMID: 8550067 PMC: 1383933.

In vitro and in vivo actions of acetylcholine receptor educated suppressor T cell lines in murine experimental autoimmune myasthenia gravis.

Pachner A, Kantor F Clin Exp Immunol. 1984; 56(3):659-68.

PMID: 6235071 PMC: 1536006.

The antibody response to specific immune complexes is under genetic control and correlates with the expression of a recurrent idiotype.

Caulfield M, Luce K, Shaffer D, Lake J J Exp Med. 1986; 163(1):75-86.

PMID: 3484514 PMC: 2188007. DOI: 10.1084/jem.163.1.75.

Antigen induces chronic idiotype suppression.

Tokuhisa T, Rajewsky K Proc Natl Acad Sci U S A. 1985; 82(12):4217-20.

PMID: 3159020 PMC: 397967. DOI: 10.1073/pnas.82.12.4217.

Detection of bacteria in the presence of antibiotics by using specific monoclonal antibodies to neutralize the antibiotics.

Sierra-Madero J, Caulfield M, Hall G, Washington J J Clin Microbiol. 1988; 26(9):1904-6.

PMID: 3053780 PMC: 266747. DOI: 10.1128/jcm.26.9.1904-1906.1988.

References

Henry C, Jerne N . Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968; 128(1):133-52. PMC: 2138503. DOI: 10.1084/jem.128.1.133. View

BOYLE W . An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968; 6(6):761-4. DOI: 10.1097/00007890-196809000-00002. View

RITTENBERG M, PRATT K . Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969; 132(2):575-81. DOI: 10.3181/00379727-132-34264. View

Feldmann M, Diener E . Antibody-mediated suppression of the immune response in vitro. I. Evidence for a central effect. J Exp Med. 1970; 131(2):247-74. PMC: 2138797. DOI: 10.1084/jem.131.2.247. View

Henney C, Ishizaka K . Studies on the immunogenicity of antigen-antibody precipitates. II. The suppressive effect of anti-carrier and anti-hapten antibodies on the immunogenicity of dinitrophenylated human gamma G globulin. J Immunol. 1970; 104(6):1540-9. View

Potter M, Lieberman R . Common individual antigenic determinants in five of eight BALB-c IgA myeloma proteins that bind phosphoryl choline. J Exp Med. 1970; 132(4):737-51. PMC: 2138855. DOI: 10.1084/jem.132.4.737. View

Strayer D, Cosenza H, Lee W, Rowley D, Kohler H . Neonatal tolerance induced by antibody against antigen-specific receptor. Science. 1974; 186(4164):640-3. DOI: 10.1126/science.186.4164.640. View

Kluskens L, Kohler H . Regulation of immune response by autogenous antibody against receptor. Proc Natl Acad Sci U S A. 1974; 71(12):5083-7. PMC: 434044. DOI: 10.1073/pnas.71.12.5083. View

CLAFLIN J, Davie J . Clonal nature of the immune response to phosphorylcholine (PC). V. Cross-idiotypic specificity among heavy chains of murine anti-PC antibodies and PC-binding myeloma proteins. J Exp Med. 1975; 141(5):1073-83. PMC: 2189783. DOI: 10.1084/jem.141.5.1073. View

10.

Sakato N, Eisen H . Antibodies to idiotypes of isologous immunoglobulins. J Exp Med. 1975; 141(6):1411-26. PMC: 2189851. DOI: 10.1084/jem.141.6.1411. View

11.

Jerne N . Towards a network theory of the immune system. Ann Immunol (Paris). 1974; 125C(1-2):373-89. View

12.

Eardley D, GERSHON R . Induction of specific suppressor T cells in vitro. J Immunol. 1976; 117(1):313-8. View

13.

Kontiainen S, Feldmann M . Suppressor cell induction in vitro. I. Kinetics of induction of antigen-specific suppressor cells. Eur J Immunol. 1976; 6(4):296-301. DOI: 10.1002/eji.1830060412. View

14.

Augustin A, Cosenza H . Expression of new idiotypes following neonatal idiotypic suppression of a dominant clone. Eur J Immunol. 1976; 6(7):497-501. DOI: 10.1002/eji.1830060710. View

15.

Mage M, McHugh L, Rothstein T . Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977; 15(1):47-56. DOI: 10.1016/0022-1759(77)90016-3. View

16.

Owen F, Ju S, Nisonoff A . Presence on idiotype-specific suppressor T cells of receptors that interact with molecules bearing the idiotype. J Exp Med. 1977; 145(6):1559-66. PMC: 2180685. DOI: 10.1084/jem.145.6.1559. View

17.

Klaus G . Antigen-antibody complexes elicit anti-idiotypic antibodies to self-idiotopes. Nature. 1978; 272(5650):265-6. DOI: 10.1038/272265a0. View

18.

Wysocki L, Sato V . "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978; 75(6):2844-8. PMC: 392661. DOI: 10.1073/pnas.75.6.2844. View

19.

LEWIS G, Goodman J . Purification of functional, determinant-specific, idiotype-bearing murine T cells. J Exp Med. 1978; 148(4):915-24. PMC: 2185016. DOI: 10.1084/jem.148.4.915. View

20.

Bona C, Paul W . Cellular basis of regulation of expression of idiotype. I. T-suppressor cells specific for MOPC 460 idiotype regulate the expression of cells secreting anti-TNP antibodies bearing 460 idiotype. J Exp Med. 1979; 149(3):592-600. PMC: 2184836. DOI: 10.1084/jem.149.3.592. View