Common Individual Antigenic Determinants in Five of Eight BALB-c IgA Myeloma Proteins That Bind Phosphoryl Choline

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1970 Oct 1

PMID 4101363

Citations 70

Authors

M Potter

R Lieberman

Affiliations

Soon will be listed here.

Abstract

Eight IgA myeloma proteins derived from independently induced plasma-cytomas in genetically similar inbred BALB/c mice are functionally related by their binding of phosphoryl choline-containing antigens (Pneumococcus C polysaccharide or Lactobacillus antigen). Each protein resembles a single species of immunoglobulin in antibody. The proteins are characterized by highly sensitive myeloma-specific antisera prepared by immunizing mice of other inbred strains with the BALB/c myeloma proteins. Individual or myeloma-specific determinants located on Fab fragments were found on three of the proteins that were unique for that protein and did not react with any other IgA protein among over 70 tested. Remarkably, five of the proteins shared two common myeloma-specific determinants which were specific for this group of five proteins. These results suggest that the five functionally and genetically related proteins sharing the same myeloma-specific determinants might also be structurally similar.

Citing Articles

Broad specificity of monoclonal IgA (TEPC15-IgA) for enteric bacteria via phosphorylcholine-mediated interaction.

Koyama S, Ito K, Usami K, Wada S, Yamashita T, Ikeda-Ohtsubo W J Vet Med Sci. 2024; 86(7):801-808.

PMID: 38839348 PMC: 11251817. DOI: 10.1292/jvms.23-0441.

Innate sensing of oxidation-specific epitopes in health and disease.

Binder C, Papac-Milicevic N, Witztum J Nat Rev Immunol. 2016; 16(8):485-97.

PMID: 27346802 PMC: 7097710. DOI: 10.1038/nri.2016.63.

IL-17 intensifies IFN-γ-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-κB.

Gao Q, Liu Y, Wu Y, Zhao Q, Wang L, Gao S Int J Mol Med. 2015; 37(2):347-58.

PMID: 26677135 PMC: 4716793. DOI: 10.3892/ijmm.2015.2433.

Atherosclerosis Susceptibility in Mice Is Independent of the V1 Immunoglobulin Heavy Chain Gene.

Centa M, Gruber S, Nilsson D, Polyzos K, Johansson D, Hansson G Arterioscler Thromb Vasc Biol. 2015; 36(1):25-36.

PMID: 26564818 PMC: 4684249. DOI: 10.1161/ATVBAHA.115.305990.

The analysis of clonal expansions in normal and autoimmune B cell repertoires.

Hershberg U, Luning Prak E Philos Trans R Soc Lond B Biol Sci. 2015; 370(1676).

PMID: 26194753 PMC: 4528416. DOI: 10.1098/rstb.2014.0239.

References

KUNKEL H . Experimental approaches tohomogenous antibody populations. iIndividual antigenic specificity, cross specificity and diversity of human antibodies. Fed Proc. 1970; 29(1):55-8. View

Brundish D, BADDILEY J . Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochem J. 1968; 110(3):573-82. PMC: 1187388. DOI: 10.1042/bj1100573. View

Rapport M, Graf L . Immunochemical reactions of lipids. Prog Allergy. 1969; 13:273-331. View

SELIGMANN M, DANON F, Basch A, Bernard J . IgG myeloma cryoglobulin with antistreptolysin activity. Nature. 1968; 220(5168):711-2. DOI: 10.1038/220711a0. View

Grey H, Mannik M, KUNKEL H . INDIVIDUAL ANTIGENIC SPECIFICITY OF MYELOMA PROTEINS. CHARACTERISTICS AND LOCALIZATION TO SUBUNITS. J Exp Med. 1965; 121:561-75. PMC: 2137982. DOI: 10.1084/jem.121.4.561. View

Potter M, Leon M . Three IgA myeloma immunoglobulins from the BALB/ mouse: precipitation with pneumococcal C polysaccharide. Science. 1968; 162(3851):369-71. DOI: 10.1126/science.162.3851.369. View

Daugharty H, Hopper J, MacDONALD A, Nisonoff A . Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. J Exp Med. 1969; 130(5):1047-62. PMC: 2180483. DOI: 10.1084/jem.130.5.1047. View

Anderson P, Potter M . Induction of plasma cell tumours in BALB-c mice with 2,6,10,14-tetramethylpentadecane (pristane). Nature. 1969; 222(5197):994-5. DOI: 10.1038/222994a0. View

Potter M . Experimental approaches to homogenous antibody populations. Mouse IgA myeloma proteins that bind polysaccharide antigens of enterobacterial origin. Fed Proc. 1970; 29(1):85-91. View

10.

Potter M, Lieberman R, Dray S . Isoantibodies specific for myeloma gamma-G and gamma-H immunoglobulins of BALB-c mice. J Mol Biol. 1966; 16(2):334-46. DOI: 10.1016/s0022-2836(66)80177-8. View

11.

OUDIN J, Michel M . Idiotypy of rabbit antibodies. I. Comparison of idiotypy of antibodies against Salmonella typhi with that of antibodies against other bacteria in the same rabbits, or of antibodies against Salmonella typhi in various rabbits. J Exp Med. 1969; 130(3):595-617. PMC: 2138715. DOI: 10.1084/jem.130.3.595. View

12.

Willims Jr R, KUNKEL H, Capra J . Antigenic specificities related to the cold agglutinin activity of gamma M globulins. Science. 1968; 161(3839):379-81. DOI: 10.1126/science.161.3839.379. View

13.

Gotschlich E, Liu T . Structural and immunological studies on the pneumococcal C polysaccharide. J Biol Chem. 1967; 242(3):463-70. View

14.

Cohn M, NOTANI G, Rice S . Characterization of the antibody to the C-carbohydrate produced by a transplantable mouse plasmacytoma. Immunochemistry. 1969; 6(1):111-23. DOI: 10.1016/0019-2791(69)90183-9. View

15.

Tomasz A . Choline in the cell wall of a bacterium: novel type of polymer-linked choline in Pneumococcus. Science. 1967; 157(3789):694-7. DOI: 10.1126/science.157.3789.694. View

16.

Potter M, BOYCE C . Induction of plasma-cell neoplasms in strain BALB/c mice with mineral oil and mineral oil adjuvants. Nature. 1962; 193:1086-7. DOI: 10.1038/1931086a0. View

17.

KELUS A, Gell P . Immunological analysis of rabbit anti-antibody systems. J Exp Med. 1968; 127(1):215-34. PMC: 2138426. DOI: 10.1084/jem.127.1.215. View