Promoter for the Unc Operon of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Sep 1

PMID 6193096

Citations 16

Authors

A C Porter

W S Brusilow

R D Simoni

Affiliations

Soon will be listed here.

Abstract

Fragments of DNA carrying possible promoters for the unc operon of Escherichia coli were cloned into a promoter detection plasmid (pRZ5255). Similar fragments were transcribed in vitro to produce transcripts whose sizes were used to determine the approximate start site for transcription. One strong promoter and at least two very much weaker ones were detected by these methods. The exact position of the strongest promoter, presumed to be the true unc promoter, was determined by S1 nuclease mapping and shown to lie 73 base pairs upstream from the open reading frame that precedes uncB. It therefore appears that this reading frame (uncI) is part of the unc operon. S1 mapping also revealed the presence of a third weak promoter 25 base pairs upstream of uncI. All of the weak promoters occur between the proposed unc promoter and uncB, but their role in vivo, if any, is unclear.

Citing Articles

The product of uncI gene in F1Fo-ATP synthase operon plays a chaperone-like role to assist c-ring assembly.

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A pathway branching in transcription initiation in Escherichia coli.

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Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny.

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Linkage map of Escherichia coli K-12, edition 10: the traditional map.

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Effects of carbon source on expression of F0 genes and on the stoichiometry of the c subunit in the F1F0 ATPase of Escherichia coli.

Schemidt R, Qu J, Williams J, Brusilow W J Bacteriol. 1998; 180(12):3205-8.

PMID: 9620972 PMC: 107823. DOI: 10.1128/JB.180.12.3205-3208.1998.

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