Cloning and Expression of UncI, the First Gene of the Unc Operon of Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Sep 1

PMID 6224772

Citations 20

Authors

W S Brusilow

A C Porter

R D Simoni

Affiliations

Soon will be listed here.

Abstract

The unc operon of Escherichia coli consists of eight genes coding for the eight subunits of the proton-translocating ATPase. In vitro transcription-translation of DNA cloned from the beginning of the operon onto plasmids reveals that the reading frame uncI, which precedes the other genes of the operon, codes for a protein with a molecular weight of 14,500, called i. In minicells, the i protein is synthesized in amounts comparable to the amounts of the ATPase subunits, suggesting that it may be part of the ATPase complex. The presence of the unc promoter and uncI on a plasmid containing the other eight genes of the unc operon has little effect on the differential expression of the unc genes or the partitioning of the newly synthesized subunits into soluble or sedimentable fractions in the in vitro system. The i protein partitions into the sedimentable fraction.

Citing Articles

Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth.

Liu J, Hicks D, Krulwich T J Bacteriol. 2012; 195(2):220-30.

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The product of uncI gene in F1Fo-ATP synthase operon plays a chaperone-like role to assist c-ring assembly.

Suzuki T, Ozaki Y, Sone N, Feniouk B, Yoshida M Proc Natl Acad Sci U S A. 2007; 104(52):20776-81.

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A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake.

Hicks D, Wang Z, Wei Y, Kent R, Guffanti A, Banciu H Proc Natl Acad Sci U S A. 2003; 100(18):10213-8.

PMID: 12917488 PMC: 193541. DOI: 10.1073/pnas.1832982100.

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

Interacting helical faces of subunits a and c in the F1Fo ATP synthase of Escherichia coli defined by disulfide cross-linking.

Jiang W, Fillingame R Proc Natl Acad Sci U S A. 1998; 95(12):6607-12.

PMID: 9618459 PMC: 22573. DOI: 10.1073/pnas.95.12.6607.

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