MCF-specific Murine Monoclonal Antibodies Made Against AKR-247 MCF Virus Recognize a Unique Determinant Associated with the Gp70-p-15(E) Complex

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Mar 1

PMID 6178839

Citations 13

Authors

M W Cloyd

B Chesebro

J L Portis

M Weir

Affiliations

Soon will be listed here.

Abstract

Hybridomas obtained from (NFS X AKR)F mice immunized with syngeneic cells infected with AKR-247 MCF virus produced antibodies specific for only AKR-247 or closely related MCF viruses which hare a previously defined MCF antigen (MCFA-3). These monoclonal antibodies recognized a new type of viral antigenic determinant which appeared to be a conformational determinant associated with the env precursor polyprotein (pr80env) or its disulfide-linked gp70-p15(E) complex (gp80) but not with free gp70 or p15(E) or any other virion or virus-induced protein.

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References

Rowe W, PUGH W, Hartley J . Plaque assay techniques for murine leukemia viruses. Virology. 1970; 42(4):1136-9. DOI: 10.1016/0042-6822(70)90362-4. View

Takatsuki A, Arima K, Tamura G . Tunicamycin, a new antibiotic. I. Isolation and characterization of tunicamycin. J Antibiot (Tokyo). 1971; 24(4):215-23. DOI: 10.7164/antibiotics.24.215. View

Sachs D, Schechter A, Eastlake A, ANFINSEN C . An immunologic approach to the conformational equilibria of polypeptides. Proc Natl Acad Sci U S A. 1972; 69(12):3790-4. PMC: 389874. DOI: 10.1073/pnas.69.12.3790. View

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

Chattopadhyay S, Lowy D, Teich N, Levine A, Rowe W . Qualitative and quantitative studies of AKR-type murine leukemia virus sequences in mouse DNA. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 2:1085-101. DOI: 10.1101/sqb.1974.039.01.124. View

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Fischinger P, Nomura S, Bolognesi D . A novel murine oncornavirus with dual eco- and xenotropic properties. Proc Natl Acad Sci U S A. 1975; 72(12):5150-5. PMC: 388894. DOI: 10.1073/pnas.72.12.5150. View

Famulari N, Buchhagen D, Klenk H, Fleissner E . Presence of murine leukemia virus envelope proteins gp70 and p15(E) in a common polyprotein of infected cells. J Virol. 1976; 20(2):501-8. PMC: 355017. DOI: 10.1128/JVI.20.2.501-508.1976. View

Cloyd M, Bigner D . Contained indirect viable-cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses. J Clin Microbiol. 1977; 5(1):86-90. PMC: 274537. DOI: 10.1128/jcm.5.1.86-90.1977. View

10.

Hartley J, Wolford N, Old L, Rowe W . A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977; 74(2):789-92. PMC: 392380. DOI: 10.1073/pnas.74.2.789. View

11.

Elder J, Gautsch J, JENSEN F, Lerner R, Hartley J, Rowe W . Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc Natl Acad Sci U S A. 1977; 74(10):4676-80. PMC: 432010. DOI: 10.1073/pnas.74.10.4676. View

12.

Rommelaere J, Faller D, Hopkins N . Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses. Proc Natl Acad Sci U S A. 1978; 75(1):495-9. PMC: 411277. DOI: 10.1073/pnas.75.1.495. View

13.

Rowe W . Leukemia virus genomes in the chromosomal DNA of the mouse. Harvey Lect. 1978; 71:173-92. View

14.

STOCKERT E, DeLeo A, ODonnell P, Obata Y, Old L . G(AKSL2): a new cell surface antigen of the mouse related to the dualtropic mink cell focus-inducing class of murine leukemia virus detected by naturally occurring antibody. J Exp Med. 1979; 149(1):200-15. PMC: 2184729. DOI: 10.1084/jem.149.1.200. View

15.

Pinter A, Lieman-Hurwitz J, Fleissner E . The nature of the association between the murine leukemia virus envelope proteins. Virology. 1978; 91(2):345-51. DOI: 10.1016/0042-6822(78)90382-3. View

16.

Cloyd M, Hartley J, Rowe W . Cell-surface antigens associated with recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1979; 149(3):702-12. PMC: 2184819. DOI: 10.1084/jem.149.3.702. View

17.

Lostrom M, Stone M, Tam M, Burnette W, Pinter A, Nowinski R . Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the p 15(E) and gp70 envelope proteins. Virology. 1979; 98(2):336-50. DOI: 10.1016/0042-6822(79)90557-9. View

18.

Cloyd M, Hartley J, Rowe W . Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980; 151(3):542-52. PMC: 2185805. DOI: 10.1084/jem.151.3.542. View

19.

Haas M, Patch V . Cell-surface antigens associated with dualtropic and thymotropic murine leukemia viruses inducing thymic and nonthymic lymphomas. J Exp Med. 1980; 151(6):1321-33. PMC: 2185874. DOI: 10.1084/jem.151.6.1321. View

20.

Keshet E, Shaul Y, Kaminchik J, Aviv H . Heterogeneity of "virus-like" genes encoding retrovirus-associated 30S RNA and their organization within the mouse genome. Cell. 1980; 20(2):431-9. DOI: 10.1016/0092-8674(80)90629-7. View