Idiotype-like Determinants on Human T Lymphocytes Alloactivated in Mixed Lymphocyte Culture

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1982 Jul 1

PMID 6177823

Citations 10

Authors

N Suciu-Foca

C Rohowsky

P Kung

D W King

Affiliations

Soon will be listed here.

Abstract

T cells alloactivated in 5-d MLC with an HLA-DR-different stimulator acquire the capacity of stimulating the autologous mixed lymphocyte response (AMLR). We have demonstrated that activation of AMLR by allosensitized T cells is determined by the expression of the idiotype receptor for the stimulating HLA-DR alloantigen. This has been shown in experiments in which purified, OKT-3-positive T cell suspensions were first primed for 9 d with AMLR-activated T lymphoblasts, then tested in secondary AMLR with autologous lymphoblasts sensitized to various HLA-DR alloantigens. Accelerated memory responses were induced only by autologous lymphoblasts that had been sensitized against the same HLA-DR specificity as the primary AMLR stimulators. This response was not inhibited by a mouse monoclonal antibody recognizing Ia-like determinants, and was not triggered by human allogeneic resting peripheral blood lymphocytes. Thus, recognition of alloactivated T lymphoblasts in secondary AMLR seems to be specific for the idiotype-like determinants expressed by the autologous stimulators.

Citing Articles

Anti-idiotypic antibodies to anti-HLA receptors induced by pregnancy.

Suciu-Foca N, Reed E, Rohowsky C, Kung P, King D Proc Natl Acad Sci U S A. 1983; 80(3):830-4.

PMID: 6600840 PMC: 393474. DOI: 10.1073/pnas.80.3.830.

Anti-idiotypic autoimmunity--a necessity for species survival.

Suciu-Foca N, Kohler H, King D Surv Immunol Res. 1984; 3(4):311-8.

PMID: 6438753 DOI: 10.1007/BF02919049.

Modulation of T-cell antigen receptor on lymphocyte membrane.

Haars R, Rohowsky-Kochan C, Reed E, King D, Suciu-Foca N Immunogenetics. 1984; 20(4):397-405.

PMID: 6333390 DOI: 10.1007/BF00345614.

Role of normal adherent cells in the regulation of the autologous mixed lymphocyte reactions in humans.

Pierri I, Rogna S, Pende D, Viglione D, Scudeletti M, Barabino A J Clin Immunol. 1984; 4(3):197-201.

PMID: 6234323 DOI: 10.1007/BF00914966.

Immunoregulatory T cell circuits in man. Alloantigen-primed inducer T cells activate alloantigen-specific suppressor T cells in the absence of the initial antigenic stimulus.

Damle N, Engleman E J Exp Med. 1983; 158(1):159-73.

PMID: 6223112 PMC: 2187074. DOI: 10.1084/jem.158.1.159.

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