Immune Response Potential to Poly(Tyr,Glu)-poly(DLAla)--poly(Lys) of Human T Cells of Different Donors

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Jul 1

PMID 6170066

Citations 8

Authors

D Katz

Z Bentwich

N Eshhar

I Lowy

E Mozes

Affiliations

Soon will be listed here.

Abstract

Human peripheral blood T cells of normal donors were activated in vitro with autologous adherent cells pulsed with poly(LTyr,LGlu)-poly(DLAla)--poly(LLys) [abbreviated (T,G)-A--L]. The "educated" T cells were tested: (i) for their ability to produce a (T,G)-A--L-specific T cell-replacing factor in the cooperation with B cells for antibody responses in vivo or in vitro and (ii) for their ability to proliferate in the presence of a second stimulus of (T,G)-A--L. Results of screening of 66 donors demonstrated that educated T cells of about 50% of the donors produced an active (T,G)-A--L-specific factor, whereas activated cells of only half of the factor producers were capable of proliferating in the presence of the antigen. Thus, as reported for all other species studied, human individuals differ in their response potential to (T,G)-A--L.

Citing Articles

Partial purification and characterization of an antigen-specific helper factor synthesized by a T-cell continuous line.

Lifshitz R, Apte R, Mozes E Proc Natl Acad Sci U S A. 1983; 80(18):5689-93.

PMID: 6225124 PMC: 384324. DOI: 10.1073/pnas.80.18.5689.

Antigen-specific HLA-restricted human T-cell lines. II. A GAT-specific T-cell line restricted by a determinant carried by an HLA-DQ molecule.

Ball E, Stastny P Immunogenetics. 1984; 20(5):547-64.

PMID: 6209215 DOI: 10.1007/BF00364357.

In vitro proliferative responses and antibody titers specific to human acetylcholine receptor synthetic peptides in patients with myasthenia gravis and relation to HLA class II genes.

Brocke S, Brautbar C, Steinman L, Abramsky O, Rothbard J, Neumann D J Clin Invest. 1988; 82(6):1894-900.

PMID: 2461962 PMC: 442769. DOI: 10.1172/JCI113807.

In vitro T-cell functions specific to an anti-DNA idiotype and serological markers in patients with systemic lupus erythematosus (SLE).

Mendlovic S, Shoenfeld Y, Bakimer R, Segal R, Dayan M, Mozes E J Clin Immunol. 1988; 8(3):178-87.

PMID: 2455730 DOI: 10.1007/BF00917564.

Analysis of antigen specific T cell helper function in first degree relatives of patients with systemic lupus erythematosus (SLE).

Segal R, Brautbar C, Katz D, Shalev Y, Bentwich Z, Mozes E Clin Exp Immunol. 1986; 66(1):52-60.

PMID: 2433083 PMC: 1542651.

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