H-2-restricted Helper Factor Secreted by Clone Hybridoma Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1981 Sep 1

PMID 6168725

Citations 8

Authors

P Lonai

J Puri

S Bitton

Y Ben-Neriah

D Givol

G J Hammerling

Affiliations

Soon will be listed here.

Abstract

Biological and serological characteristics of a helper factor secreted by cloned hybridoma cells was described. The factor is carrier specific and contains determinants shared with immunoglobulin VH bu does not react with V kappa- or V lambda-specific antibodies. Presence of four H-2I-controlled antigenic specificities, Ia.ml, Ia.m2, Ia.17, and Ia.m7, was detected. Hence, it is possible that both A beta and E alpha loci may be involved in its control. Helper effect could be obtained only toward B cell sources that shared the H-2K and I-A antigens with the hybridoma cells. Similarly, the factor was absorbed only by spleen cells syngeneic in I-A. Previous studies have demonstrated that this clone binds antigen in an H-2-restricted manner. It follows that H-2-restricted helper cells produce H-2-restricted helper factors. Hence, they support the view that specific T cell factors may represent secreted T cell receptors.

Citing Articles

Inhibition by Fab and Fab'2 monoclonal anti-Ia antibody fragments of T-lymphocyte proliferative responses.

Lemonnier F, Dubreuil P, Caillol D Immunology. 1982; 46(3):533-44.

PMID: 6980183 PMC: 1555320.

Human T cell hybridomas secreting immune interferon.

Le J, Vilcek J, Saxinger C, Prensky W Proc Natl Acad Sci U S A. 1982; 79(24):7857-61.

PMID: 6818554 PMC: 347448. DOI: 10.1073/pnas.79.24.7857.

Characterization of a human X mouse T cell hybridoma and identification of a clone secreting and binding interleukin-2.

Durrant L, Parkar M, Kenworthy N, Taylor G Immunology. 1984; 52(1):117-28.

PMID: 6609121 PMC: 1454574.

The role of products of the human HLA-DR locus (Ia molecules) in in vitro M. leprae driven lymphoproliferation.

Haregewoin A, Yemaneberhan T, Belehu A Clin Exp Immunol. 1983; 53(2):328-34.

PMID: 6349878 PMC: 1535697.

Retention and loss of immunoglobulin heavy chain alleles in helper T cell hybridoma clones.

Lonai P, Rechavi G, Arman E, Givol D EMBO J. 1983; 2(5):781-6.

PMID: 6227480 PMC: 555185. DOI: 10.1002/j.1460-2075.1983.tb01500.x.

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